論文 - 詳細
| RRC ID | 44199 |
|---|---|
| 著者 | Nakayama F, Umeda S, Ichimiya T, Kamiyama S, Hazawa M, Yasuda T, Nishihara S, Imai T. |
| タイトル | Sulfation of keratan sulfate proteoglycan reduces radiation-induced apoptosis in human Burkitt's lymphoma cell lines. |
| ジャーナル | FEBS Lett |
| Abstract |
This study focuses on clarifying the contribution of sulfation to radiation-induced apoptosis in human Burkitt's lymphoma cell lines, using 3'-phosphoadenosine 5'-phosphosulfate transporters (PAPSTs). Overexpression of PAPST1 or PAPST2 reduced radiation-induced apoptosis in Namalwa cells, whereas the repression of PAPST1 expression enhanced apoptosis. Inhibition of PAPST slightly decreased keratan sulfate (KS) expression, so that depletion of KS significantly increased radiation-induced apoptosis. In addition, the repression of all three N-acetylglucosamine-6-O-sulfotransferases (CHST2, CHST6, and CHST7) increased apoptosis. In contrast, PAPST1 expression promoted the phosphorylation of p38 MAPK and Akt in irradiated Namalwa cells. These findings suggest that 6-O-sulfation of GlcNAc residues in KS reduces radiation-induced apoptosis of human Burkitt's lymphoma cells. |
| 巻・号 | 587(2) |
| ページ | 231-7 |
| 公開日 | 2013-1-16 |
| DOI | 10.1016/j.febslet.2012.12.002 |
| PII | S0014-5793(12)00904-0 |
| PMID | 23238079 |
| MeSH | Anion Transport Proteins / antagonists & inhibitors Anion Transport Proteins / genetics Anion Transport Proteins / metabolism Apoptosis / physiology Apoptosis / radiation effects Base Sequence Burkitt Lymphoma / metabolism* Burkitt Lymphoma / pathology Burkitt Lymphoma / radiotherapy Cell Line, Tumor Humans Keratan Sulfate / chemistry Keratan Sulfate / metabolism* MAP Kinase Signaling System Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism Proteoglycans / chemistry Proteoglycans / metabolism* RNA, Small Interfering / genetics Radiation Tolerance / physiology Sulfate Transporters Sulfuric Acid Esters / metabolism |
| IF | 3.057 |
| 引用数 | 8 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Raji Daudi |