Reference - Detail
|Author||Nakayama F, Umeda S, Ichimiya T, Kamiyama S, Hazawa M, Yasuda T, Nishihara S, Imai T.|
|Title||Sulfation of keratan sulfate proteoglycan reduces radiation-induced apoptosis in human Burkitt's lymphoma cell lines.|
This study focuses on clarifying the contribution of sulfation to radiation-induced apoptosis in human Burkitt's lymphoma cell lines, using 3'-phosphoadenosine 5'-phosphosulfate transporters (PAPSTs). Overexpression of PAPST1 or PAPST2 reduced radiation-induced apoptosis in Namalwa cells, whereas the repression of PAPST1 expression enhanced apoptosis. Inhibition of PAPST slightly decreased keratan sulfate (KS) expression, so that depletion of KS significantly increased radiation-induced apoptosis. In addition, the repression of all three N-acetylglucosamine-6-O-sulfotransferases (CHST2, CHST6, and CHST7) increased apoptosis. In contrast, PAPST1 expression promoted the phosphorylation of p38 MAPK and Akt in irradiated Namalwa cells. These findings suggest that 6-O-sulfation of GlcNAc residues in KS reduces radiation-induced apoptosis of human Burkitt's lymphoma cells.
|MeSH||Anion Transport Proteins / antagonists & inhibitors Anion Transport Proteins / genetics Anion Transport Proteins / metabolism Apoptosis / physiology Apoptosis / radiation effects Base Sequence Burkitt Lymphoma / metabolism* Burkitt Lymphoma / pathology Burkitt Lymphoma / radiotherapy Cell Line, Tumor Humans Keratan Sulfate / chemistry Keratan Sulfate / metabolism* MAP Kinase Signaling System Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism Proteoglycans / chemistry Proteoglycans / metabolism* RNA, Small Interfering / genetics Radiation Tolerance / physiology Sulfate Transporters Sulfuric Acid Esters / metabolism|
|WOS Category||BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|
|Human and Animal Cells|