論文 - 詳細
| RRC ID | 4421 |
|---|---|
| 著者 | Anzai D, Tonoyama Y, Ikeda A, Kawasaki T, Oka S. |
| タイトル | Regulated expression of the HNK-1 carbohydrate is essential for medaka (Oryzias latipes) embryogenesis. |
| ジャーナル | Glycobiology |
| Abstract |
Carbohydrates are known to play essential roles in various biological processes including development. However, it remains largely unknown which carbohydrate structure takes part in each biological event. Here, we examined the roles of the human natural killer-1 (HNK-1) carbohydrate in medaka embryogenesis. We first cloned two medaka glucuronyltransferases, GlcAT-P and GlcAT-S, key enzymes for HNK-1 biosynthesis. Overexpression of these glucuronyltransferases affected morphogenetic processes. In addition, loss-of-function experiments revealed that GlcAT-P is physiologically indispensable for head morphogenesis and GlcAT-P depletion also led to markedly increased apoptosis. However, even when the apoptosis was blocked, abnormal head morphogenesis caused by GlcAT-P depletion was still observed, indicating that apoptosis was not the main cause of the abnormality. Moreover, in situ hybridization analyses indicated that GlcAT-P depletion resulted in the abnormal formation of the nervous system but not in cell specification. These results suggest that tight regulation of HNK-1 expression is essential for proper morphogenesis of medaka embryos. |
| 巻・号 | 19(8) |
| ページ | 868-78 |
| 公開日 | 2009-8-1 |
| DOI | 10.1093/glycob/cwp060 |
| PII | cwp060 |
| PMID | 19389918 |
| MeSH | Amino Acid Sequence Animals Apoptosis / physiology CD57 Antigens / biosynthesis* Embryo, Nonmammalian / physiology Glucuronosyltransferase / metabolism* Molecular Sequence Data Oryzias / embryology* Oryzias / metabolism |
| IF | 4.06 |
| 引用数 | 3 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | HdrR-II1 (IB178) cDNA |