Reference - Detail
| RRC ID | 44210 |
|---|---|
| Author | Futamura Y, Kawatani M, Kazami S, Tanaka K, Muroi M, Shimizu T, Tomita K, Watanabe N, Osada H. |
| Title | Morphobase, an encyclopedic cell morphology database, and its use for drug target identification. |
| Journal | Chem Biol |
| Abstract |
Visual observation is a powerful approach for screening bioactive compounds that can facilitate the discovery of attractive druggable targets following their chemicobiological validation. So far, many high-content approaches, using sophisticated imaging technology and bioinformatics, have been developed. In our study, we aimed to develop a simpler method that focuses on intact cell images because we found that dynamic changes in morphology are informative, often reflecting the mechanism of action of a drug. Here, we constructed a chemical-genetic phenotype profiling system, based on the high-content cell morphology database Morphobase. This database compiles the phenotypes of cancer cell lines that are induced by hundreds of reference compounds, wherein those of well-characterized anticancer drugs are classified by mode of action. Furthermore, we demonstrate the applicability of this system in identifying NPD6689, NPD8617, and NPD8969 as tubulin inhibitors. |
| Volume | 19(12) |
| Pages | 1620-30 |
| Published | 2012-12-21 |
| DOI | 10.1016/j.chembiol.2012.10.014 |
| PII | S1074-5521(12)00415-2 |
| PMID | 23261605 |
| MeSH | Animals Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology* Cell Line, Tumor Cytological Techniques / methods Databases, Factual* Drug Discovery / methods* Humans Image Processing, Computer-Assisted / methods Neoplasms / drug therapy* Neoplasms / pathology* Small Molecule Libraries / chemistry Small Molecule Libraries / pharmacology* |
| IF | 5.915 |
| Times Cited | 56 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | NRK(RCB0043) |