論文 - 詳細
| RRC ID | 44214 |
|---|---|
| 著者 | Xue FM, Zhang HP, Hao HJ, Shi ZY, Zhou C, Feng B, Yang PC. |
| タイトル | CD98 positive eosinophils contribute to T helper 1 pattern inflammation. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND AND AIMS:The pathogenesis of inflammatory bowel disease (IBD) has not been fully understood yet. Eosinophils (Eo) are one type of the major proinflammatory cells of the chronic inflammation in the intestine. CD98 is involved in the pathogenesis of a number of inflammations. This study aims to elucidate the role of CD98(+) Eos in the initiation of intestinal inflammation. METHODS:The colon biopsies were collected from 60 patients with IBD. The expression of CD98 in the biopsies was examined by immunohistochemistry. The serum levels of the flagellin (FGN) antibody and Eo-derived mediators in the culture supernatants were assessed by enzyme-linked immunosorbent assay. The role of FGN on Eo activation was examined in a cell culture model. The role of FGN in the induction of colitis was observed in a mouse model. RESULTS:Compared to normal controls, the frequency of CD98(+) Eos was markedly increased in the IBD colon mucosa. FGN were detected in the colon biopsies and in the sera of IBD patients. Exposure to FGN induced the expression of galectin 3 (the ligand of CD98) in dendritic cells. The exposure to galectin 3 activated the CD98(+) Eos. After treatment with FGN intrarectally, mice with eosinophilia showed severe inflammation in the colon. CONCLUSIONS:The interaction of galectin 3 and CD98 can induce Eos to release chemical mediators that contributes to the initiation of the intestinal inflammation. |
| 巻・号 | 7(12) |
| ページ | e51830 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0051830 |
| PII | PONE-D-12-22424 |
| PMID | 23272174 |
| PMC | PMC3521696 |
| MeSH | Adolescent Adult Aged Animals Cell Line Colitis / chemically induced Colitis / metabolism Colitis / pathology Dendritic Cells / immunology Dendritic Cells / metabolism Disease Models, Animal Eosinophils / immunology* Eosinophils / metabolism* Female Flagellin / adverse effects Flagellin / metabolism Fusion Regulatory Protein-1 / metabolism* Galectin 3 / metabolism Humans Inflammation / immunology* Inflammation / metabolism* Inflammation / pathology Inflammatory Bowel Diseases / immunology Inflammatory Bowel Diseases / metabolism Inflammatory Bowel Diseases / pathology Intestinal Mucosa / immunology Intestinal Mucosa / metabolism Intestinal Mucosa / pathology Male Mice Middle Aged T-Lymphocytes, Helper-Inducer / immunology* Young Adult |
| IF | 2.74 |
| 引用数 | 7 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | EoL-1 cell(RCB0641) |