論文 - 詳細
| RRC ID | 44233 |
|---|---|
| 著者 | Kikuchi T, Pan X, Ishii K, Nakamura Y, Ogihara E, Koike K, Tanaka R, Akihisa T. |
| タイトル | Cytotoxic and apoptosis-inducing activities of 12-O-Acetylazedarachin B from the fruits of Melia azedarach in human cancer cell lines. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
12-O-Acetylazedarachin B (1), isolated from the fruit extract of Melia azedarach, exhibited potent cytotoxicity against leukemia (HL-60) (IC(50) 0.016 µM) and stomach (AZ521) (IC(50) 0.035 µM) cancer cell lines. Upon assessing the apoptosis-inducing activity in HL-60 cells, compound 1 exhibited induction of apoptosis detected by the observation of membrane phospholipid exposure and DNA fragmentation in flow cytometry. Western blot analysis showed that 1 markedly reduced the levels of procaspases-3, 8, and 9, while being increased the levels of cleaved caspases-3, 8, and 9. In addition, compound 1 increased significantly Bax/Bcl-2 ratio. These results suggested that 1 induced apoptotic cell death in HL-60 via both mitochondrial and death receptor-mediated pathways. Therefore, compound 1 may be promising lead compound for developing an effective drug for treatment of leukemia. Flow cytometric analysis suggested that the cytotoxicity of 1 against AZ521 is due to inducing apoptosis as well as necrosis with the latter predominated. |
| 巻・号 | 36(1) |
| ページ | 135-9 |
| 公開日 | 2013-1-1 |
| DOI | 10.1248/bpb.b12-00795 |
| PII | DN/JST.JSTAGE/bpb/b12-00795 |
| PMID | 23302647 |
| MeSH | Antineoplastic Agents / pharmacology* Apoptosis / drug effects Cell Cycle / drug effects Cell Line, Tumor Cell Survival / drug effects Fruit Humans Limonins / isolation & purification Limonins / pharmacology* Melia azedarach* Plant Extracts / pharmacology |
| IF | 1.863 |
| 引用数 | 16 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) A549(RCB0098) SK-BR-3(RCB2132) |