論文 - 詳細
| RRC ID | 44245 |
|---|---|
| 著者 | Kawaguchi T, Komatsu S, Ichikawa D, Morimura R, Tsujiura M, Konishi H, Takeshita H, Nagata H, Arita T, Hirajima S, Shiozaki A, Ikoma H, Okamoto K, Ochiai T, Taniguchi H, Otsuji E. |
| タイトル | Clinical impact of circulating miR-221 in plasma of patients with pancreatic cancer. |
| ジャーナル | Br J Cancer |
| Abstract |
BACKGROUND:Several recent studies have demonstrated that microRNAs (miRNAs) are stably detectable in plasma/serum. We tested miR-221 and miR-375, which are frequently reported to be highly and poorly expressed in pancreatic cancer (PCa), as candidates for plasma biomarkers in PCa. METHODS:This study was divided into three parts: (1) Confirmation of higher miR-221 levels in primary PCa tissue and cell lines than normal pancreatic tissues. (2) Evaluation of plasma miR-221 and miR-375 concentrations by comparing results from 47 consecutive PCa patients and 30 healthy volunteers. (3) Evaluation of the assay for monitoring tumour dynamics in PCa patients. RESULTS:(1) Expression of miR-221 was significantly higher in PCa tissues and cell lines than normal pancreatic tissues. (2) Plasma miR-221 concentrations were significantly higher in PCa patients than that in benign pancreatic tumours (P=0.016) and controls (P<0.0005), while plasma miR-375 concentrations tended to be lower in PCa patients (P=0.064), and the miR-221/miR-375 ratio was significantly higher (P<0.0001) in PCa patients than in controls. (3) Plasma miR-221 concentrations were significantly reduced in postoperative samples (P=0.018). Furthermore, PCa patients with high plasma miR-221 concentrations had significant correlation with distant metastasis (P=0.041), and non-resectable status (P=0.021). CONCLUSION:Plasma miR-221 could be a useful biomarker for cancer detection, monitoring tumour dynamics and predicting malignant outcomes in PCa patients, and may contribute to clinical decision making in PCa treatments. |
| 巻・号 | 108(2) |
| ページ | 361-9 |
| 公開日 | 2013-2-5 |
| DOI | 10.1038/bjc.2012.546 |
| PII | bjc2012546 |
| PMID | 23329235 |
| PMC | PMC3566805 |
| MeSH | Aged Biomarkers, Tumor / blood Biomarkers, Tumor / genetics Cell Line, Tumor Female Humans Male MicroRNAs / blood* Pancreatic Neoplasms / blood* Pancreatic Neoplasms / genetics* |
| IF | 5.791 |
| 引用数 | 136 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | PK-45H(RCB1973) PANC-1(RCB2095) PK-59(RCB1901) KP4-1(RCB1006) PK-1(RCB1972) |