論文 - 詳細
| RRC ID | 44260 |
|---|---|
| 著者 | Ishii G, Hashimoto H, Atsumi N, Hoshino A, Ochiai A. |
| タイトル | Morphophenotype of floating colonies derived from a single cancer cell has a critical impact on tumor-forming activity. |
| ジャーナル | Pathol Int |
| Abstract |
The anchorage-independent colony growth of cancer cells is reportedly correlated with the tumor-forming activity; however, the correlation between the morphophenotype of each colony and the tumor-forming activity has not been clarified. To assess this problem, we cultured single A549 cells (human lung adenocarcinoma cell line) in growth medium in individual wells (n = 426) for 14 days under anchorage-independent conditions and analyzed the resulting growth characteristics. The single A549 cells formed various sizes of floating colonies. The proportion of large colonies (>400 μm) was 3.8% and this proportion increased dramatically with the exogenous addition of EGF (21.6%) or HGF (27.6%). Morphologically, the floating colonies could be divided into: (ii) Type A, spheroid colony; and (ii) Type B, dispersed villous colony. The Type B colonies expressed significantly higher levels of epithelial-mesenchymal transition (EMT)-related mRNAs (Snail 1, ZEB 1, and ZEB2) than the Type A colonies. Furthermore, the subcutaneous injection of a single cell-derived colony with a large size and a Type B morphology resulted in more efficient tumor formation. The present results indicated that the morphophenotypes of floating colonies derived from single cancer cells have a critical impact on tumor-forming activity. |
| 巻・号 | 63(1) |
| ページ | 29-36 |
| 公開日 | 2013-1-1 |
| DOI | 10.1111/pin.12018 |
| PMID | 23356223 |
| MeSH | Adenocarcinoma / genetics Adenocarcinoma / pathology* Animals Cell Adhesion Cell Adhesion Molecules Cell Count Cell Line, Tumor Epithelial-Mesenchymal Transition* Female Humans Lung Neoplasms / genetics Lung Neoplasms / pathology* Mice Mice, SCID Neoplasm Invasiveness Neoplasm Transplantation Neoplastic Cells, Circulating / pathology* Single-Cell Analysis Spheroids, Cellular Tumor Stem Cell Assay |
| IF | 2.11 |
| 引用数 | 2 |
| WOS 分野 | PATHOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |