RRC ID 44263
Author Raju R, Jian B, Hooks JJ, Nagineni CN.
Title Transforming growth factor-β regulates the expression of anosmin (KAL-1) in human retinal pigment epithelial cells.
Journal Cytokine
Abstract In a microarray analysis of human retinal pigment epithelial cells (HRPE) treated with TGF-β, in addition to the alteration of a number of known Extracellular matrix (ECM)-related genes regulated by TGF-β, we found a significant increase in the expression of Kallmann Syndrome (KAL)-1 gene, that codes for the protein anosmin-1. Enhanced expression of KAL-1 by TGF-β was validated by real-time PCR analysis. In in vitro experiments, TGF-β receptor inhibitor abolished TGF-β-induced expression of KAL-1. Immunofluorescence staining showed increased presence of anosmin-1 in TGF-β treated HRPE cells, with distinct localization at the intercellular junctions. Treatment of HRPE cells with TGF-β enhanced secretion of anosmin-1 and the release of anosmin-1 was further augmented by heparin sulfate. Enhanced secretion of anosmin-1 in the presence of TGF-β and heparin was also observed in other ocular cells such as corneal epithelial and corneal fibroblast cultures. The role of anosmin-1, a protein with adhesion functions, in retinal structure, function and pathology has not been known and remains to be investigated.
Volume 61(3)
Pages 724-7
Published 2013-3-1
DOI 10.1016/j.cyto.2012.12.019
PII S1043-4666(12)00829-0
PMID 23357298
PMC PMC3595383
MeSH Epithelial Cells / drug effects Epithelial Cells / metabolism* Extracellular Matrix Proteins / genetics* Extracellular Matrix Proteins / metabolism Fluorescent Antibody Technique Gene Expression Regulation / drug effects* Humans Immunoblotting Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism Pigment Epithelium of Eye / cytology* Real-Time Polymerase Chain Reaction Transforming Growth Factor beta / pharmacology*
IF 2.952
Times Cited 5
Human and Animal Cells HCE-T(RCB2280)