論文 - 詳細
| RRC ID | 44263 |
|---|---|
| 著者 | Raju R, Jian B, Hooks JJ, Nagineni CN. |
| タイトル | Transforming growth factor-β regulates the expression of anosmin (KAL-1) in human retinal pigment epithelial cells. |
| ジャーナル | Cytokine |
| Abstract |
In a microarray analysis of human retinal pigment epithelial cells (HRPE) treated with TGF-β, in addition to the alteration of a number of known Extracellular matrix (ECM)-related genes regulated by TGF-β, we found a significant increase in the expression of Kallmann Syndrome (KAL)-1 gene, that codes for the protein anosmin-1. Enhanced expression of KAL-1 by TGF-β was validated by real-time PCR analysis. In in vitro experiments, TGF-β receptor inhibitor abolished TGF-β-induced expression of KAL-1. Immunofluorescence staining showed increased presence of anosmin-1 in TGF-β treated HRPE cells, with distinct localization at the intercellular junctions. Treatment of HRPE cells with TGF-β enhanced secretion of anosmin-1 and the release of anosmin-1 was further augmented by heparin sulfate. Enhanced secretion of anosmin-1 in the presence of TGF-β and heparin was also observed in other ocular cells such as corneal epithelial and corneal fibroblast cultures. The role of anosmin-1, a protein with adhesion functions, in retinal structure, function and pathology has not been known and remains to be investigated. |
| 巻・号 | 61(3) |
| ページ | 724-7 |
| 公開日 | 2013-3-1 |
| DOI | 10.1016/j.cyto.2012.12.019 |
| PII | S1043-4666(12)00829-0 |
| PMID | 23357298 |
| PMC | PMC3595383 |
| MeSH | Epithelial Cells / drug effects Epithelial Cells / metabolism* Extracellular Matrix Proteins / genetics* Extracellular Matrix Proteins / metabolism Fluorescent Antibody Technique Gene Expression Regulation / drug effects* Humans Immunoblotting Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism Pigment Epithelium of Eye / cytology* Real-Time Polymerase Chain Reaction Transforming Growth Factor beta / pharmacology* |
| IF | 2.952 |
| 引用数 | 5 |
| WOS 分野 | IMMUNOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |