RRC ID 44269
Author Mori N, Ohno J, Sakagami R, Hayakawa T, Fukushima T.
Title Cell viabilities and biodegradation rates of DNA/protamine complexes with two different molecular weights of DNA.
Journal J Biomed Mater Res B Appl Biomater
Abstract Two types of DNA/protamine complexes were prepared from protamine sulfate and 7000 base pair (bp) DNA or original DNA to investigate the effect of the molecular weight of DNA on zeta potential, cell viability, flowability, soft tissue response, and biodegradation rate. The 7000 bp DNA/protamine complex had a negative charge while the original DNA/protamine complex had a positive charge. The cell viabilities (90.4-106.8%) of these complexes were close to each other. The 7000 bp DNA/protamine complex became a softer dough than that of the original DNA/complex when both were kneaded with water. In vivo, the original DNA/protamine complex showed a milder tissue response. The original DNA/protamine complex almost disappeared 30 days after implantation. The 7000 bp DNA/complex disappeared approximately 2 weeks after implantation and areas where samples were implanted became empty. Thereafter, the empty space was gradually replaced by new soft tissues. The original DNA/protamine complex showed low intercalation and groove binding ratios of daunorubicin hydrochloride. Results indicate that high DNA condensation by cationic protamine protected the penetration of degradation enzymes into these complexes. It was found that a high molecular weight of DNA reduced the biodegradation rate and flowability. This study suggests that DNA/protamine complexes could be candidates for biomaterials that control biodegradation rates and flowability.
Volume 101(5)
Pages 743-51
Published 2013-7-1
DOI 10.1002/jbm.b.32877
PMID 23359567
MeSH 3T3 Cells Absorbable Implants Animals Biocompatible Materials / chemistry* Biocompatible Materials / metabolism Cell Survival DNA / chemistry* DNA / metabolism Daunorubicin / metabolism Male Materials Testing Mice Molecular Weight Osteoblasts / cytology Osteoblasts / metabolism Protamines / chemistry* Protamines / metabolism Rats Rats, Sprague-Dawley Solubility Surface Properties Tissue Engineering
IF 2.674
Times Cited 10
Human and Animal Cells