| RRC ID |
44269
|
| 著者 |
Mori N, Ohno J, Sakagami R, Hayakawa T, Fukushima T.
|
| タイトル |
Cell viabilities and biodegradation rates of DNA/protamine complexes with two different molecular weights of DNA.
|
| ジャーナル |
J Biomed Mater Res B Appl Biomater
|
| Abstract |
Two types of DNA/protamine complexes were prepared from protamine sulfate and 7000 base pair (bp) DNA or original DNA to investigate the effect of the molecular weight of DNA on zeta potential, cell viability, flowability, soft tissue response, and biodegradation rate. The 7000 bp DNA/protamine complex had a negative charge while the original DNA/protamine complex had a positive charge. The cell viabilities (90.4-106.8%) of these complexes were close to each other. The 7000 bp DNA/protamine complex became a softer dough than that of the original DNA/complex when both were kneaded with water. In vivo, the original DNA/protamine complex showed a milder tissue response. The original DNA/protamine complex almost disappeared 30 days after implantation. The 7000 bp DNA/complex disappeared approximately 2 weeks after implantation and areas where samples were implanted became empty. Thereafter, the empty space was gradually replaced by new soft tissues. The original DNA/protamine complex showed low intercalation and groove binding ratios of daunorubicin hydrochloride. Results indicate that high DNA condensation by cationic protamine protected the penetration of degradation enzymes into these complexes. It was found that a high molecular weight of DNA reduced the biodegradation rate and flowability. This study suggests that DNA/protamine complexes could be candidates for biomaterials that control biodegradation rates and flowability.
|
| 巻・号 |
101(5)
|
| ページ |
743-51
|
| 公開日 |
2013-7-1
|
| DOI |
10.1002/jbm.b.32877
|
| PMID |
23359567
|
| MeSH |
3T3 Cells
Absorbable Implants
Animals
Biocompatible Materials / chemistry*
Biocompatible Materials / metabolism
Cell Survival
DNA / chemistry*
DNA / metabolism
Daunorubicin / metabolism
Male
Materials Testing
Mice
Molecular Weight
Osteoblasts / cytology
Osteoblasts / metabolism
Protamines / chemistry*
Protamines / metabolism
Rats
Rats, Sprague-Dawley
Solubility
Surface Properties
Tissue Engineering
|
| IF |
2.831
|
| 引用数 |
10
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
|
