| RRC ID |
44294
|
| 著者 |
Hoshino T, Mabuchi K.
|
| タイトル |
Closed-looped in situ nano processing on a culturing cell using an inverted electron beam lithography system.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
The beam profile of an electron beam (EB) can be focused onto less than a nanometer spot and scanned over a wide field with extremely high speed sweeping. Thus, EB is employed for nano scale lithography in applied physics research studies and in fabrication of semiconductors. We applied a scanning EB as a control system for a living cell membrane which is representative of large scale complex systems containing nanometer size components. First, we designed the opposed co-axial dual optics containing inverted electron beam lithography (I-EBL) system and a fluorescent optical microscope. This system could provide in situ nano processing for a culturing living cell on a 100-nm-thick SiN nanomembrane, which was placed between the I-EBL and the fluorescent optical microscope. Then we demonstrated the EB-induced chemical direct nano processing for a culturing cell with hundreds of nanometer resolution and visualized real-time images of the scanning spot of the EB-induced luminescent emission and chemical processing using a high sensitive camera mounted on the optical microscope. We concluded that our closed-loop in situ nano processing would be able to provide a nanometer resolution display of virtual molecule environments to study functional changes of bio-molecule systems.
|
| 巻・号 |
432(2)
|
| ページ |
345-9
|
| 公開日 |
2013-3-8
|
| DOI |
10.1016/j.bbrc.2013.01.100
|
| PII |
S0006-291X(13)00190-3
|
| PMID |
23396058
|
| MeSH |
Cell Culture Techniques*
Computer Simulation / statistics & numerical data
Electrons
Hep G2 Cells
Humans
Luminescence
Microscopy, Fluorescence
Monte Carlo Method
Nanostructures
Nanotechnology / methods*
|
| IF |
2.985
|
| 引用数 |
12
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
