| RRC ID |
44305
|
| Author |
Kondoh T, Kuribayashi K, Tanaka M, Kobayashi D, Yanagihara N, Watanabe N.
|
| Title |
CD7 promotes extramedullary involvement of the B-cell acute lymphoblastic leukemia line Tanoue by enhancing integrin β2-dependent cell adhesiveness.
|
| Journal |
Int J Oncol
|
| Abstract |
Extramedullary involvement (EMI) is a factor that defines prognosis of acute lymphoblastic leukemia; however, the molecular mechanism(s) remain elusive. Here, we show that CD7 promotes EMI of the human B-cell acute lymphoblastic leukemia cell line Tanoue. The Tanoue cell line expressing firefly luciferase, Luc-Tanoue, was transplanted into non-obese diabetic/severe combined immunodeficient mice, and cells infiltrated into the brain were cultured ex vivo. This process was repeated 4 times to obtain the highly invasive line Luc-Tanoue-F4. Comparison of the global gene expression signatures of Luc-Tanoue-F4 and Luc-Tanoue indicated that the CD7 gene showed the largest increase in expression among EMI-related genes in Luc-Tanoue-F4 cells. Overexpression of CD7 in Tanoue enhanced cell invasiveness. Among cell migration, proliferation, adhesion and protease activity, only cell adhesiveness showed enhancement in Luc-Tanoue-F4. Expression of the intracellular domain, but not the extracellular domain, of CD7 enhanced cell adhesiveness. Luc-Tanoue-F4 showed a higher level of integrin β2 expression; overexpression of CD7 induced the expression of integrin β2 in Luc-Tanoue. These results show that CD7 induces integrin β2 and enhances cell adhesiveness and invasiveness in Tanoue cells. This study highlights the role of the CD7/integrin β2 axis as a critical pathway in the process of EMI of human B-cell acute lymphoblastic leukemia.
|
| Volume |
45(3)
|
| Pages |
1073-81
|
| Published |
2014-9-1
|
| DOI |
10.3892/ijo.2014.2492
|
| PMID |
24920488
|
| MeSH |
Animals
CD18 Antigens / metabolism*
Cell Adhesion
Cell Line, Tumor
Female
Gene Expression Regulation, Neoplastic
Humans
Mice
Mice, Inbred NOD
Neoplasm Invasiveness / genetics*
Neoplasms, Experimental
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / metabolism*
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / pathology*
|
| IF |
3.147
|
| Times Cited |
19
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
TRANSPLANTATION
CELL & TISSUE ENGINEERING
HEMATOLOGY
|
| Resource |
| Human and Animal Cells |
Tanoue |
