Reference - Detail
| RRC ID | 44316 |
|---|---|
| Author | Tiwari A, Sharma Y, Hattori S, Terada D, Sharma AK, Turner AP, Kobayashi H. |
| Title | Influence of poly(n-isopropylacrylamide)-CNT-polyaniline three-dimensional electrospun microfabric scaffolds on cell growth and viability. |
| Journal | Biopolymers |
| Abstract |
This study investigates the effect on: (1) the bulk surface and (2) the three-dimensional non-woven microfabric scaffolds of poly(N-isopropylacrylamide)-CNT-polyaniline on growth and viability of cells. The poly(N-isopropylacrylamide)-CNT-polyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, non-woven microfabric scaffolds. The electrospun microfabrics were assembled in regular three-dimensional scaffolds with OD: 400-500 μm; L: 6-20 cm. Mice fibroblast cells L929 were seeded on the both poly(N-isopropylacrylamide)-CNT-polyaniline bulk surface as well as non-woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N-isopropylacrylamide)-CNT-polyaniline non-woven microfabric matrices in compare to poly(N-isopropylacrylamide)-CNT-polyaniline bulk and commercially available Matrigel™ even with a range of cell lines up to 168 h. Temperature dependent cells detachment behavior was observed on the poly(N-isopropylacrylamide)-CNT-polyaniline scaffolds by varying incubation at below lower critical solution temperature of poly(N-isopropylacrylamide). The results suggest that poly(N-isopropylacrylamide)-CNT-polyaniline non-woven microfabrics could be used as a smart matrices for applications in tissue engineering. |
| Volume | 99(5) |
| Pages | 334-41 |
| Published | 2013-5-1 |
| DOI | 10.1002/bip.22170 |
| PMID | 23426576 |
| MeSH | Acrylic Resins / chemistry* Aniline Compounds / chemistry* Animals Cell Adhesion Cell Culture Techniques / methods Cell Proliferation* Cell Survival Collagen Drug Combinations Electrochemical Techniques / methods Fibroblasts / cytology L Cells Laminin Magnetic Resonance Spectroscopy Mice Microscopy, Electron, Scanning Polymers / chemistry* Proteoglycans Temperature Time Factors Tissue Engineering / methods* Tissue Scaffolds / chemistry* |
| IF | 1.854 |
| Times Cited | 30 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | L929 |