| RRC ID |
44321
|
| Author |
Mashima H, Ohno H, Yamada Y, Sakai T, Ohnishi H.
|
| Title |
INSL5 may be a unique marker of colorectal endocrine cells and neuroendocrine tumors.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Insulin-like peptide 5 (INSL5) is a member of the insulin superfamily, and is a potent agonist for RXFP4. We have shown that INSL5 is expressed in enteroendocrine cells (EECs) along the colorectum with a gradient increase toward the rectum. RXFP4 is ubiquitously expressed along the digestive tract. INSL5-positive EECs have little immunoreactivity to chromogranin A (CgA) and might be a unique marker of colorectal EECs. CgA-positive EECs were distributed normally along the colorectum in INSL5 null mice, suggesting that INSL5 is not required for the development of CgA-positive EECs. Exogenous INSL5 did not affect the proliferation of human colon cancer cell lines, and chemically-induced colitis in INSL5 null mice did not show any significant changes in inflammation or mucosal healing compared to wild-type mice. In contrast, all of the rectal neuroendocrine tumors examined co-expressed INSL5 and RXFP4. INSL5 may be a unique marker of colorectal EECs, and INSL5-RXFP4 signaling might play a role in an autocrine/paracrine fashion in the colorectal epithelium and rectal neuroendocrine tumors.
|
| Volume |
432(4)
|
| Pages |
586-92
|
| Published |
2013-3-22
|
| DOI |
10.1016/j.bbrc.2013.02.042
|
| PII |
S0006-291X(13)00302-1
|
| PMID |
23438439
|
| MeSH |
Animals
Autocrine Communication
Biomarkers / metabolism
Biomarkers, Tumor / metabolism
Cell Line, Tumor
Colon / metabolism*
Colorectal Neoplasms / metabolism
Enteroendocrine Cells / metabolism*
Humans
Insulin / genetics
Insulin / metabolism*
Mice
Mice, Inbred C57BL
Mice, Knockout
Neuroendocrine Tumors / metabolism
Paracrine Communication
Proteins / genetics
Proteins / metabolism*
Receptors, G-Protein-Coupled / metabolism*
Receptors, Peptide / metabolism*
Rectum / metabolism*
|
| IF |
2.985
|
| Times Cited |
17
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
CACO-2(RCB0988)
LoVo(RCB1639) |
