RRC ID 44342
Author Lin S, Hirai S, Goto T, Sakamoto T, Takahashi N, Yano M, Sasaki T, Yu R, Kawada T.
Title Auraptene suppresses inflammatory responses in activated RAW264 macrophages by inhibiting p38 mitogen-activated protein kinase activation.
Journal Mol Nutr Food Res
Abstract SCOPE:Inflammation plays a key role in obesity-related pathologies such as insulin resistance and type 2 diabetes. Hypertrophied adipocytes trigger the enhancement of macrophage infiltration and the release of various proinflammatory factors in obese adipose tissue. In this study, we examined whether auraptene, a citrus-fruit-derived compound, could suppress the production of inflammatory factors that mediate the interaction between adipocytes and macrophages.
METHODS AND RESULTS:Experiments using a co-culture system of 3T3-L1 adipocytes and RAW264 macrophages showed that auraptene reduced the production of nitric oxide and tumor necrosis factor-α. In RAW264 macrophages, auraptene also suppressed the inflammation induced by either LPS or the conditioned medium derived from 3T3-L1 adipocytes. In addition, auraptene inhibited the phosphorylation of the p38 mitogen-activated protein kinase and suppressed the production of proinflammatory mediators in activated macrophages.
CONCLUSION:Our findings indicate that auraptene exhibits anti-inflammatory properties by suppressing the production of inflammatory factors that mediate the interaction between adipocytes and macrophages, suggesting that auraptene is a valuable food-derived compound with a potential to attenuate chronic inflammation in adipose tissue and to improve obesity-related insulin resistance.
Volume 57(7)
Pages 1135-44
Published 2013-7
DOI 10.1002/mnfr.201200611
PMID 23495198
MeSH 3T3-L1 Cells Adipocytes / drug effects Adipocytes / metabolism Adipose Tissue / drug effects Adipose Tissue / metabolism Animals Anti-Inflammatory Agents / pharmacology* Cell Line, Tumor Chemokine CCL2 / metabolism Citrus / chemistry Coculture Techniques Coumarins / pharmacology* Culture Media, Conditioned Fruit / chemistry Inflammation / drug therapy Inflammation / pathology Insulin Resistance Lipopolysaccharides / adverse effects Macrophages / drug effects* Macrophages / metabolism Mice Nitric Oxide / antagonists & inhibitors Nitric Oxide / metabolism Obesity / metabolism Phosphorylation Plant Extracts / pharmacology* Tumor Necrosis Factor-alpha / antagonists & inhibitors Tumor Necrosis Factor-alpha / biosynthesis p38 Mitogen-Activated Protein Kinases / genetics* p38 Mitogen-Activated Protein Kinases / metabolism
IF 4.653
Times Cited 15
Human and Animal Cells