Reference - Detail
|Author||Lin S, Hirai S, Goto T, Sakamoto T, Takahashi N, Yano M, Sasaki T, Yu R, Kawada T.|
|Title||Auraptene suppresses inflammatory responses in activated RAW264 macrophages by inhibiting p38 mitogen-activated protein kinase activation.|
|Journal||Mol Nutr Food Res|
SCOPE:Inflammation plays a key role in obesity-related pathologies such as insulin resistance and type 2 diabetes. Hypertrophied adipocytes trigger the enhancement of macrophage infiltration and the release of various proinflammatory factors in obese adipose tissue. In this study, we examined whether auraptene, a citrus-fruit-derived compound, could suppress the production of inflammatory factors that mediate the interaction between adipocytes and macrophages.
METHODS AND RESULTS:Experiments using a co-culture system of 3T3-L1 adipocytes and RAW264 macrophages showed that auraptene reduced the production of nitric oxide and tumor necrosis factor-α. In RAW264 macrophages, auraptene also suppressed the inflammation induced by either LPS or the conditioned medium derived from 3T3-L1 adipocytes. In addition, auraptene inhibited the phosphorylation of the p38 mitogen-activated protein kinase and suppressed the production of proinflammatory mediators in activated macrophages.
CONCLUSION:Our findings indicate that auraptene exhibits anti-inflammatory properties by suppressing the production of inflammatory factors that mediate the interaction between adipocytes and macrophages, suggesting that auraptene is a valuable food-derived compound with a potential to attenuate chronic inflammation in adipose tissue and to improve obesity-related insulin resistance.
|MeSH||3T3-L1 Cells Adipocytes / drug effects Adipocytes / metabolism Adipose Tissue / drug effects Adipose Tissue / metabolism Animals Anti-Inflammatory Agents / pharmacology* Cell Line, Tumor Chemokine CCL2 / metabolism Citrus / chemistry Coculture Techniques Coumarins / pharmacology* Culture Media, Conditioned Fruit / chemistry Inflammation / drug therapy Inflammation / pathology Insulin Resistance Lipopolysaccharides / adverse effects Macrophages / drug effects* Macrophages / metabolism Mice Nitric Oxide / antagonists & inhibitors Nitric Oxide / metabolism Obesity / metabolism Phosphorylation Plant Extracts / pharmacology* Tumor Necrosis Factor-alpha / antagonists & inhibitors Tumor Necrosis Factor-alpha / biosynthesis p38 Mitogen-Activated Protein Kinases / genetics* p38 Mitogen-Activated Protein Kinases / metabolism|
|WOS Category||FOOD SCIENCE & TECHNOLOGY|
|Human and Animal Cells|