論文 - 詳細
RRC ID | 44342 |
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著者 | Lin S, Hirai S, Goto T, Sakamoto T, Takahashi N, Yano M, Sasaki T, Yu R, Kawada T. |
タイトル | Auraptene suppresses inflammatory responses in activated RAW264 macrophages by inhibiting p38 mitogen-activated protein kinase activation. |
ジャーナル | Mol Nutr Food Res |
Abstract |
SCOPE:Inflammation plays a key role in obesity-related pathologies such as insulin resistance and type 2 diabetes. Hypertrophied adipocytes trigger the enhancement of macrophage infiltration and the release of various proinflammatory factors in obese adipose tissue. In this study, we examined whether auraptene, a citrus-fruit-derived compound, could suppress the production of inflammatory factors that mediate the interaction between adipocytes and macrophages. METHODS AND RESULTS:Experiments using a co-culture system of 3T3-L1 adipocytes and RAW264 macrophages showed that auraptene reduced the production of nitric oxide and tumor necrosis factor-α. In RAW264 macrophages, auraptene also suppressed the inflammation induced by either LPS or the conditioned medium derived from 3T3-L1 adipocytes. In addition, auraptene inhibited the phosphorylation of the p38 mitogen-activated protein kinase and suppressed the production of proinflammatory mediators in activated macrophages. CONCLUSION:Our findings indicate that auraptene exhibits anti-inflammatory properties by suppressing the production of inflammatory factors that mediate the interaction between adipocytes and macrophages, suggesting that auraptene is a valuable food-derived compound with a potential to attenuate chronic inflammation in adipose tissue and to improve obesity-related insulin resistance. |
巻・号 | 57(7) |
ページ | 1135-44 |
公開日 | 2013-7-1 |
DOI | 10.1002/mnfr.201200611 |
PMID | 23495198 |
MeSH | 3T3-L1 Cells Adipocytes / drug effects Adipocytes / metabolism Adipose Tissue / drug effects Adipose Tissue / metabolism Animals Anti-Inflammatory Agents / pharmacology* Cell Line, Tumor Chemokine CCL2 / metabolism Citrus / chemistry Coculture Techniques Coumarins / pharmacology* Culture Media, Conditioned Fruit / chemistry Inflammation / drug therapy Inflammation / pathology Insulin Resistance Lipopolysaccharides / adverse effects Macrophages / drug effects* Macrophages / metabolism Mice Nitric Oxide / antagonists & inhibitors Nitric Oxide / metabolism Obesity / metabolism Phosphorylation Plant Extracts / pharmacology* Tumor Necrosis Factor-alpha / antagonists & inhibitors Tumor Necrosis Factor-alpha / biosynthesis p38 Mitogen-Activated Protein Kinases / genetics* p38 Mitogen-Activated Protein Kinases / metabolism |
IF | 5.309 |
引用数 | 15 |
WOS 分野 | FOOD SCIENCE & TECHNOLOGY |
リソース情報 | |
ヒト・動物細胞 | RAW 264(RCB0535) |