論文 - 詳細
| RRC ID | 44363 |
|---|---|
| 著者 | Wang X, Li X, Ito A, Sogo Y, Ohno T. |
| タイトル | Particle-size-dependent toxicity and immunogenic activity of mesoporous silica-based adjuvants for tumor immunotherapy. |
| ジャーナル | Acta Biomater |
| Abstract |
Conventionally used adjuvants alone are insufficient for triggering cell-mediated immunity, although they have been successfully developed to elicit protective antibody responses in some vaccines. Here, with the aim of eliciting cell-mediated immunity, pathogen-associated molecular patterns (PAMPs) were immobilized with apatite within the pores and on the surface of mesoporous silica (MS) with particle sizes from 30 to 200nm to prepare novel MS-Ap-PAMP adjuvants, which showed cell-mediated anti-tumor immunity that was markedly improved compared to commercial alum adjuvant in vitro and in vivo. The toxicity and antitumor immunity of the MS-Ap-PAMP adjuvants were evaluated in vitro and in vivo. MS with a particle size of 200nm showed minimum in vitro cytotoxicity to NIH3T3 cells, particularly at concentrations no higher than 100μgml(-1). In particular, apatite precipitation within the pores and on the surface of MS decreased the in vitro cytotoxicity of MS particles. The MS-Ap-PAMP adjuvants showed the maximum in vitro immunogenic activity among original culture medium, PAMP and alum-PAMP. Moreover, injection of the MS-Ap-PAMP adjuvant in combination with liquid-nitrogen-treated tumor tissue (derived from Lewis lung carcinoma cells) into C57BL/6 mice markedly inhibited in vivo tumor recurrence and the development of rechallenged tumor compared to those with commercial alum adjuvant. The MS-Ap-PAMP adjuvant contributed to the elicitation of a potent systemic antitumor immunity without obvious toxicity in vivo. |
| 巻・号 | 9(7) |
| ページ | 7480-9 |
| 公開日 | 2013-7-1 |
| DOI | 10.1016/j.actbio.2013.03.031 |
| PII | S1742-7061(13)00158-X |
| PMID | 23541601 |
| MeSH | Adjuvants, Immunologic / therapeutic use* Animals Cancer Vaccines / immunology* Cancer Vaccines / therapeutic use* Carcinoma, Lewis Lung / immunology* Carcinoma, Lewis Lung / therapy* Cell Line, Tumor Cell Survival / drug effects Mice Mice, Inbred C57BL NIH 3T3 Cells Particle Size Porosity Silicon Dioxide / immunology* Silicon Dioxide / therapeutic use* Treatment Outcome |
| IF | 7.242 |
| 引用数 | 39 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) |