| RRC ID |
44412
|
| 著者 |
Maeda Y, Hojo H, Shimohata N, Choi S, Yamamoto K, Takato T, Chung UI, Ohba S.
|
| タイトル |
Bone healing by sterilizable calcium phosphate tetrapods eluting osteogenic molecules.
|
| ジャーナル |
Biomaterials
|
| Abstract |
Although bone grafts and prosthetic implants have shown some clinical success in the treatment of bone defects, the graft availability, biocompatibility, function, and longevity still remain to be improved. One possible solution to these problems is to develop bone implants acting on host cells to induce rapid bone regeneration. Here, we demonstrate bone healing by means of a sterilizable and osteogenic molecule-eluting implant system in which two small molecules, a smoothened agonist (SAG) and a helioxanthin derivative (TH), are loaded onto tetrapod-shaped calcium phosphate granules (Tetrabone). We succeeded in directing progenitor cells toward mature osteoblasts with the combined application of the two small molecules acting on different stages of osteogenesis. Tetrabone released SAG and TH for prolonged periods when loaded with these molecules. EOG sterilization did not affect the osteogenic activity of the SAG- and TH-loaded Tetrabones. The combinatorial use of SAG- and TH-loaded Tetrabones achieved bone healing without cell transplantation in a rat femur bone defect model within two weeks. This system will allow us to vary the combination rate of implants loaded with different osteogenic factors depending on the types and sizes of defects, potentially allowing full temporal and spatial control of the bone regeneration.
|
| 巻・号 |
34(22)
|
| ページ |
5530-7
|
| 公開日 |
2013-7-1
|
| DOI |
10.1016/j.biomaterials.2013.03.089
|
| PII |
S0142-9612(13)00423-7
|
| PMID |
23623228
|
| MeSH |
Animals
Calcium Phosphates / pharmacology*
Cell Line
Femur / diagnostic imaging
Femur / drug effects*
Femur / pathology*
Implants, Experimental
Lignans / pharmacology
Male
Mesoderm / cytology
Mice
Osteogenesis / drug effects*
Radiography
Rats
Rats, Wistar
Sterilization*
Time Factors
Wound Healing / drug effects*
|
| IF |
10.317
|
| 引用数 |
14
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126)
10T1/2(RCB0247) |
