RRC ID 44417
Author Yasunaga M, Masui E, Oji A, Soma A, Osaki M, Nakanishi T, Sato K.
Title Identification of the control region of pancreatic expression of Bmp4 in vitro and in vivo.
Journal PLoS ONE
Abstract Bone morphogenetic protein 4 (Bmp4) was recently shown to be related to glucose homeostasis in mouse adult pancreas through the regulation of insulin production. We previously revealed the predominant expression of Bmp4 in adult pancreas by in vivo imaging of transgenic mice. However, the control regions for predominant Bmp4 expression in the adult pancreas are unclear. In this study, we established transgenic (Tg) mice that allow real time in vivo bioluminescence imaging of the enhancer/promoter activity of the Bmp4 gene. Tg mice expressing firefly luciferase with a 7 kb upstream region and 5'-non-coding sequence (three exons and two introns) of the Bmp4 gene showed pancreatic expression of bioluminescence, while the Tg mice bearing luciferase with the 7 kb upstream region alone did not show pancreatic expression of the reporter gene. Interestingly, pancreatic expression of bioluminescence was also present in Tg mice harboring the truncated promoter without exon IA and IB, indicating the presence of a cryptic promoter in front of exon II. Furthermore, the bioluminescence signal was not detected in embryonic pancreas, but increasing signals were observed in neonatal and infantile Tg mice depending on the genotypes observed. These results suggested that a novel mechanism of transcription is involved in pancreatic expression of the Bmp4 gene.
Volume 8(4)
Pages e61821
Published 2013
DOI 10.1371/journal.pone.0061821
PII PONE-D-12-34996
PMID 23626735
PMC PMC3633997
MeSH Animals Animals, Newborn Bone Morphogenetic Protein 4 / genetics* Bone Morphogenetic Protein 4 / metabolism Embryo, Mammalian Exons Gene Expression Regulation, Developmental* Genes, Reporter Introns Luciferases Luminescent Measurements Mice Mice, Transgenic Microscopy, Fluorescence NIH 3T3 Cells Pancreas / growth & development Pancreas / metabolism* Promoter Regions, Genetic* RNA, Messenger / genetics* RNA, Messenger / metabolism Transcription, Genetic*
IF 2.776
Times Cited 1
Human and Animal Cells