RRC ID 44426
著者 Kato S, Saitoh Y, Miwa N.
タイトル Inhibitions by hydrogen-occluding silica microcluster to melanogenesis in human pigment cells and tyrosinase reaction.
ジャーナル J Nanosci Nanotechnol
Abstract We investigated the anti-melanogenetic efficacy of hydrogen-occluding silica microcluster (H2-Silica), which is a silsesquioxane-based compound with hydrogen interstitially embedded in a matrix of caged silica, against melanogenesis in HMV-II human melanoma cells and L-DOPA-tyrosinase reaction [EC1.14.18.1]. HMV-II cells were subjected to oxidative stress by ultraviolet ray-A (UVA) exposure of 3-times of 0.65 J/cm2 summed up to 1.95 J/cm2. After UVA irradiation, HMV-II cells were stimulated to produce melanin by 2.72-fold more abundantly than unirradiated control. When HMV-II cells were treated with H2-Silica of 20 ppm or kojic acid of 28.4 ppm before and after UVA-irradiation, the amount of melanin was repressed to 12.2% or 14.5% as compared to that of UVA-irradiated control, respectively. That is, H2-Silica exhibited a comparable efficacy to the whitening agent kojic acid. The H2-Silica could prevent melanogenesis in HMV-II cells by low-level doses at 1-10 ppm, and cell viability and apoptosis event did not change even by high-level doses at 100-1000 ppm. On the contrary, kojic acid was cytotoxic at the concentration of 14-28 ppm or more. By microscopic observation, H2-Silica suppressed such properties indicative of melanin-rich cells as cellular hypertrophy, cell process formation, and melanogenesis around the outside of nuclei. The enzymatic assay using L-DOPA and mushroom tyrosinase demonstrated that H2-Silica restrained UVA-mediated melanin formation owing to down-regulation of tyrosinase activity, which could be attributed to scavenging of free radicals and inhibition of L-DOPA-to-dopachrome oxidation by hydrogen released from H2-Silica. Thus H2-Silica has a potential to prevent melanin production against UVA and serves as a skin-lightening ingredient for supplements or cosmetics.
巻・号 13(1)
ページ 52-9
公開日 2013-1-1
DOI 10.1166/jnn.2013.6848
PMID 23646697
MeSH Capsules / administration & dosage Cell Line, Tumor Cell Survival / drug effects Humans Hydrogen / administration & dosage* Melanoma / enzymology Melanoma / etiology* Melanoma / prevention & control* Monophenol Monooxygenase / metabolism* Neoplasms, Radiation-Induced / enzymology Neoplasms, Radiation-Induced / etiology* Neoplasms, Radiation-Induced / prevention & control* Silicon Dioxide / administration & dosage* Treatment Outcome Ultraviolet Rays / adverse effects
IF 1.134
引用数 6
WOS 分野 CHEMISTRY, MULTIDISCIPLINARY PHYSICS, APPLIED PHYSICS, CONDENSED MATTER MATERIALS SCIENCE, MULTIDISCIPLINARY NANOSCIENCE & NANOTECHNOLOGY
リソース情報
ヒト・動物細胞 HMV-II(RCB0777)