論文 - 詳細
| RRC ID | 44429 |
|---|---|
| 著者 | Wang X, Li X, Ito A, Sogo Y, Ohno T. |
| タイトル | Pore size-dependent immunogenic activity of mesoporous silica-based adjuvants in cancer immunotherapy. |
| ジャーナル | J Biomed Mater Res A |
| Abstract |
Commonly used aluminum hydroxide (Alum) adjuvant provokes a strong type 2 helper T cell (Th2) response for mediating antibody production but is rather ineffective for disease prevention that requires type 1 helper T cell (Th1) response for mediating cellular immunity in human vaccination. Here, for the purpose of inducing Th1 antitumor immunity, a mesoporous silica (MS)-based adjuvant is prepared. Three kinds of MS particles with nearly identical particle size and surface area but different pore sizes of 4, 7 and 10 nm were prepared. No serious in vitro cytotoxicity was observed for the MS particles at 5, 20, 50, and 100 μg/mL. Pathogen-associated molecular patterns (PAMPs) were immobilized with apatite (Ap) on MS to prepare the MS-based and PAMP-loaded adjuvants (MS-Ap-PAMP adjuvants). Macrophage-like cells cultured in the presence of MS-Ap-PAMP adjuvant with a MS pore size of 10 nm showed the maximum in vitro immunogenic activity. Injection of the MS-Ap-PAMP adjuvant with a MS pore size of 10 nm in combination with liquid nitrogen-treated tumor tissue (derived from Lewis lung carcinoma cells) to C57BL/6 mice markedly inhibited the development of rechallenged tumor in vivo, while no such antitumor immunity was induced in injection of Alum mixed with PAMP in combination with liquid nitrogen-treated tumor tissue. The MS-Ap-PAMP adjuvant contributed to the elicitation of a potent systemic Th1 antitumor immunity in vivo. |
| 巻・号 | 102(4) |
| ページ | 967-74 |
| 公開日 | 2014-4-1 |
| DOI | 10.1002/jbm.a.34783 |
| PMID | 23650285 |
| MeSH | Adjuvants, Immunologic / pharmacology Adjuvants, Immunologic / therapeutic use* Adsorption Animals Apatites / chemistry Carcinoma, Lewis Lung / drug therapy Carcinoma, Lewis Lung / immunology Cell Survival / drug effects Granulocyte-Macrophage Colony-Stimulating Factor / metabolism Humans Immunotherapy* Macrophages / drug effects Macrophages / metabolism Mice Mice, Inbred C57BL Microscopy, Electron, Scanning NIH 3T3 Cells Neoplasms / drug therapy* Neoplasms / immunology* Nitrogen Porosity Silicon Dioxide / chemistry* Spectroscopy, Fourier Transform Infrared X-Ray Diffraction |
| IF | 3.525 |
| 引用数 | 14 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LLC(RCB0558) |