| RRC ID |
44429
|
| 著者 |
Wang X, Li X, Ito A, Sogo Y, Ohno T.
|
| タイトル |
Pore size-dependent immunogenic activity of mesoporous silica-based adjuvants in cancer immunotherapy.
|
| ジャーナル |
J Biomed Mater Res A
|
| Abstract |
Commonly used aluminum hydroxide (Alum) adjuvant provokes a strong type 2 helper T cell (Th2) response for mediating antibody production but is rather ineffective for disease prevention that requires type 1 helper T cell (Th1) response for mediating cellular immunity in human vaccination. Here, for the purpose of inducing Th1 antitumor immunity, a mesoporous silica (MS)-based adjuvant is prepared. Three kinds of MS particles with nearly identical particle size and surface area but different pore sizes of 4, 7 and 10 nm were prepared. No serious in vitro cytotoxicity was observed for the MS particles at 5, 20, 50, and 100 μg/mL. Pathogen-associated molecular patterns (PAMPs) were immobilized with apatite (Ap) on MS to prepare the MS-based and PAMP-loaded adjuvants (MS-Ap-PAMP adjuvants). Macrophage-like cells cultured in the presence of MS-Ap-PAMP adjuvant with a MS pore size of 10 nm showed the maximum in vitro immunogenic activity. Injection of the MS-Ap-PAMP adjuvant with a MS pore size of 10 nm in combination with liquid nitrogen-treated tumor tissue (derived from Lewis lung carcinoma cells) to C57BL/6 mice markedly inhibited the development of rechallenged tumor in vivo, while no such antitumor immunity was induced in injection of Alum mixed with PAMP in combination with liquid nitrogen-treated tumor tissue. The MS-Ap-PAMP adjuvant contributed to the elicitation of a potent systemic Th1 antitumor immunity in vivo.
|
| 巻・号 |
102(4)
|
| ページ |
967-74
|
| 公開日 |
2014-4-1
|
| DOI |
10.1002/jbm.a.34783
|
| PMID |
23650285
|
| MeSH |
Adjuvants, Immunologic / pharmacology
Adjuvants, Immunologic / therapeutic use*
Adsorption
Animals
Apatites / chemistry
Carcinoma, Lewis Lung / drug therapy
Carcinoma, Lewis Lung / immunology
Cell Survival / drug effects
Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
Humans
Immunotherapy*
Macrophages / drug effects
Macrophages / metabolism
Mice
Mice, Inbred C57BL
Microscopy, Electron, Scanning
NIH 3T3 Cells
Neoplasms / drug therapy*
Neoplasms / immunology*
Nitrogen
Porosity
Silicon Dioxide / chemistry*
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction
|
| IF |
3.525
|
| 引用数 |
14
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
LLC(RCB0558) |
