論文 - 詳細
| RRC ID | 44451 |
|---|---|
| 著者 | Maruyama M, Yamashita Y, Kase M, Trifonov S, Sugimoto T. |
| タイトル | Lineage-specific purification of neural stem/progenitor cells from differentiated mouse induced pluripotent stem cells. |
| ジャーナル | Stem Cells Transl Med |
| Abstract |
Since induced pluripotent stem (iPS) cells have differentiation potential into all three germ layer-derived tissues, efficient purification of target cells is required in many fields of iPS research. One useful strategy is isolation of desired cells from differentiated iPS cells by lineage-specific expression of a drug-resistance gene, followed by drug selection. With this strategy, we purified neural stem/progenitor cells (NSCs), a good candidate source for regenerative therapy, from differentiated mouse iPS cells. We constructed a bicistronic expression vector simultaneously expressing blasticidin S resistance gene and DsRed under the control of tandem enhancer of a 257-base pair region of nestin second intron, an NSC-specific enhancer. This construct was efficiently inserted into the iPS genome by piggyBac transposon-mediated gene transfer, and the established subclone was differentiated into NSCs in the presence or absence of blasticidin S. Consequently, incubation with blasticidin S led to purification of NSCs from differentiated iPS cells. Our results suggest that a lineage-specific drug selection strategy is useful for purification of NSCs from differentiated iPS cells and that this strategy can be applied for the purification of other cell types. |
| 巻・号 | 2(6) |
| ページ | 420-33 |
| 公開日 | 2013-6-1 |
| DOI | 10.5966/sctm.2012-0139 |
| PII | sctm.2012-0139 |
| PMID | 23694811 |
| PMC | PMC3673754 |
| MeSH | Animals Cell Differentiation Cell Line Cell Lineage / drug effects Cell Lineage / genetics* Cell Separation / methods* Fetal Proteins / genetics Fetal Proteins / metabolism Gene Expression / drug effects* Genes, Reporter Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism* Intermediate Filament Proteins / genetics* Intermediate Filament Proteins / metabolism Introns Luciferases Mice Microscopy, Fluorescence Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism Nestin Neural Stem Cells / cytology Neural Stem Cells / drug effects Neural Stem Cells / metabolism* Nucleosides / pharmacology RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism SOXB1 Transcription Factors / genetics SOXB1 Transcription Factors / metabolism T-Box Domain Proteins / genetics T-Box Domain Proteins / metabolism Transfection |
| IF | 6.429 |
| 引用数 | 4 |
| WOS 分野 | CELL & TISSUE ENGINEERING |
| リソース情報 | |
| ヒト・動物細胞 | iPS-MEF-Ng-20D-17(APS0001) |