RRC ID 44455
Author Kibe T, Fuchigami T, Kishida M, Iijima M, Ishihata K, Hijioka H, Miyawaki A, Semba I, Nakamura N, Kiyono T, Kishida S.
Title A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis.
Journal Oral Surg Oral Med Oral Pathol Oral Radiol
Abstract OBJECTIVE:Ameloblastoma has a high risk of bone invasion and local recurrence. However, the mechanisms of bone invasion in ameloblastoma remain unclear. In this study, we established an experimental model for matrix metalloproteinase (MMP) induction and osteoclastogenesis using ameloblastoma-derived cells.
STUDY DESIGN:We established an ameloblastoma-derived cell line without viral genes and analyzed the expression of all Wnt and Frizzled members and MMPs by real-time reverse transcription-polymerase chain reaction, and analyzed the activity of MMP-2 and MMP-9 by the in-gel-gelatinase assay.
RESULTS:AM-3, newly established ameloblastoma-derived cells retained the morphology of primary-cultured ameloblastoma cells. AM-3 cells overexpressed the messenger RNA of Wnt-5a, Frizzled-2, MMP-2, and MMP-9 and showed the potential of osteoclastogenesis. In addition, Wnt-3a-treatment induced expression and activation of MMP-9 in AM-3 cells.
CONCLUSIONS:Our study suggests that AM-3 cells retained the characteristics of ameloblastoma, without acquiring typical features of cancer cells. Furthermore, Wnt signaling induced MMP-9 in ameloblastoma cells.
Volume 115(6)
Pages 780-8
Published 2013-6-1
DOI 10.1016/j.oooo.2013.03.005
PII S2212-4403(13)00141-7
PMID 23706918
MeSH Ameloblastoma / metabolism* Cell Line, Tumor / metabolism Epithelial-Mesenchymal Transition / genetics Epithelial-Mesenchymal Transition / physiology* Frizzled Receptors / genetics Frizzled Receptors / metabolism* Gene Expression Humans Jaw Neoplasms / genetics Jaw Neoplasms / metabolism* Matrix Metalloproteinase 9 / metabolism* Osteoclasts / metabolism* RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Wnt Signaling Pathway / physiology*
IF 1.601
Times Cited 12
DNA material CSII-CMV-RfA (RDB04386)