RRC ID 44483
Author Hashimoto M, Toshima H, Yonezawa T, Kawai K, Narushima T, Kaga M, Endo K.
Title Responses of RAW264.7 macrophages to water-dispersible gold and silver nanoparticles stabilized by metal-carbon σ-bonds.
Journal J Biomed Mater Res A
Abstract Nanometals are currently receiving considerable attention for industrial and biomedical applications, but their potentially hazardous and toxic effects have not been extensively studied. This study evaluated the biological responses of novel water-dispersible gold (Au-NPs) and silver nanoparticles (Ag-NPs) stabilized by Au-C or Ag-C σ-bonds in cultured macrophages (RAW264.7), via analysis of the cell viability, the integrity of the plasma membrane, and the inflammatory and morphological properties. The cultured RAW264.7 was exposed to metal-NPs at various concentrations. The Ag-NPs showed cytotoxicity at high NP concentrations, but the cytotoxic effects of the Au-NPs were smaller than those of the Ag-NPs. For the microscopic analysis, both types of particles were internalized into cells, the morphological changes in the cells which manifested as an expansion of the vesicles' volume, were smaller for the Au-NPs compared with the Ag-NPs. For the Ag-NPs, the endocytosis abilities of the macrophages might have induced harmful effects, because of the expansion of the cell vesicles. Although an inflammatory response was observed for both the Au- and Ag-NPs, the harmful effects of the Au-NPs were smaller than those of the Ag-NPs, with minor morphological changes observed even after internalization of the NPs into the cells.
Volume 102(6)
Pages 1838-49
Published 2014-6
DOI 10.1002/jbm.a.34854
PMID 23784947
MeSH Animals Carbon / chemistry Carbon / pharmacology Cell Line Cell Survival / drug effects Gold / chemistry* Gold / pharmacology* Macrophages / cytology Macrophages / drug effects* Macrophages / immunology Mice Nanoparticles / chemistry* Nanoparticles / metabolism Silver / chemistry* Silver / pharmacology* Solubility Water / chemistry
IF 3.221
Times Cited 16
Human and Animal Cells