| RRC ID |
44487
|
| 著者 |
Takei T, Sugihara K, Yoshida M, Kawakami K.
|
| タイトル |
Injectable and biodegradable sugar beet pectin/gelatin hydrogels for biomedical applications.
|
| ジャーナル |
J Biomater Sci Polym Ed
|
| Abstract |
Injectable hydrogels have advantages over pre-formed hydrogels in biomedical applications. In our previous study, we showed usefulness of sugar beet pectin (SBP) as an injectable gel material. However, the in vivo biodegradability of the gels was low because animals lack suitable hydrolytic enzymes of SBP. In this study we developed SBP-based injectable gels with higher in vivo biodegradability than the previous SBP gels by incorporating biodegradable gelatin into the latter. An aqueous solution with dissolved SBP and gelatin rapidly (< 1 min) formed gels through a horseradish peroxidase-catalyzed oxidative coupling reaction between feruloyl moieties on the SBP molecules and phenolic moieties on the gelatin molecules. Gelation time and mechanical properties of the gels were tunable by adjusting the polymer concentrations. The gels containing doxorubicin, an anti-cancer drug, successfully suppressed the growth of a solid tumor created by subcutaneous injection of mouse melanoma B16F1 cells into nude mice. These results indicate that injectable and biodegradable SBP/gelatin gels are useful in biomedical applications.
|
| 巻・号 |
24(11)
|
| ページ |
1333-42
|
| 公開日 |
2013-1-1
|
| DOI |
10.1080/09205063.2012.757727
|
| PMID |
23796034
|
| MeSH |
Absorbable Implants
Animals
Beta vulgaris / chemistry*
Biomedical Technology / methods*
Cell Line, Tumor
Doxorubicin / administration & dosage
Drug Carriers / administration & dosage
Drug Carriers / chemical synthesis*
Drug Carriers / chemistry
Drug Carriers / pharmacokinetics
Gelatin / administration & dosage*
Gelatin / chemistry
Gelatin / pharmacokinetics
Hydrogels / administration & dosage*
Hydrogels / chemistry
Hydrogels / pharmacokinetics
Injections
Male
Melanoma, Experimental / drug therapy
Melanoma, Experimental / pathology
Mice
Mice, Inbred BALB C
Mice, Nude
Pectins / administration & dosage*
Pectins / chemistry
Pectins / pharmacokinetics
Skin Neoplasms / drug therapy
Skin Neoplasms / pathology
|
| IF |
2.69
|
| 引用数 |
17
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
POLYMER SCIENCE
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
B16F1(RCB2649) |
