Reference - Detail
| RRC ID | 44490 |
|---|---|
| Author | Okumura S, Ishikawa T, Saitoh H, Akao T, Mizuki E. |
| Title | Identification of a second cytotoxic protein produced by Bacillus thuringiensis A1470. |
| Journal | Biotechnol Lett |
| Abstract |
Bacillus thuringiensis A1470 produces multiple proteins with similar molecular masses (~30 kDa) with cytotoxicity against human cell lines. One that was previously identified, parasporin-4, is a β-pore-forming toxin. The N-terminal sequence of a second cytotoxic protein was identical to a partial sequence of parasporin-2 produced by B. thuringiensis A1547. PCR was performed on total plasmid DNA from A1470 by using primers for parasporin-2 to amplify a gene which was then cloned. The cloned gene differed from A1547 parasporin-2 by 8 bp and the predicted protein differed by four amino acids. The gene was expressed in Escherichia coli, and the cytotoxic activities of the recombinant protein against four human cell lines (MOLT-4, Jurkat, HeLa, and HepG2) were similar to those of A1547 parasporin-2. We then confirmed that the A1470 strain simultaneously produces parasporin-2 and parasporin-4. |
| Volume | 35(11) |
| Pages | 1889-94 |
| Published | 2013-11-1 |
| DOI | 10.1007/s10529-013-1275-6 |
| PMID | 23801126 |
| MeSH | Bacillus thuringiensis / genetics Bacillus thuringiensis / metabolism* Bacterial Toxins / genetics Bacterial Toxins / metabolism* Cell Line Cloning, Molecular Cytotoxins / genetics Cytotoxins / metabolism* DNA, Bacterial / chemistry DNA, Bacterial / genetics Escherichia coli / genetics Gene Expression Humans Molecular Sequence Data Plasmids Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / toxicity Sequence Analysis, DNA |
| IF | 1.977 |
| Times Cited | 7 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MOLT-4(RCB0206) Jurkat HeLa Hep G2 |