| RRC ID |
44496
|
| 著者 |
Hanazaki Y, Masumoto J, Sato S, Furusawa K, Fukui A, Sasaki N.
|
| タイトル |
Multiscale analysis of changes in an anisotropic collagen gel structure by culturing osteoblasts.
|
| ジャーナル |
ACS Appl Mater Interfaces
|
| Abstract |
Mimicking the complicated anisotropic structures of a native tissue is extremely important in tissue engineering. In a previous study, we developed an anisotropic collagen gel scaffold (ACGS) having a hierarchical structure and a properties gradient. In this study, our objective was to see how cells remodel the scaffolds through the cells-ACGS interaction. For this purpose, we cultured osteoblastic cells on ACGS, which we regarded as a model system for the cells-extracellular matrix (cell-ECM) interaction. Changes in the ACGS-cell composites structure by cell-ECM interactions was investigated from a macroscopic level to a microscopic level. Osteoblastic cells were also cultured on an isotropic collagen gel (ICGS) as a control. During the cultivation, mechanical stimuli were applied to collagen-cell composites for adequate matrix remodeling. Confocal laser scanning microscope (CLSM) was used to observe macroscopic changes in the ACGS-cell composite structure by osteoblastic cells. Small-angle X-ray scattering (SAXS) measurements were performed to characterize microscopic structural changes in the composites. Macroscopic observations using CLSM revealed that osteoblastic cells remained only in the diluted phase in ACGS and they collected collagen fibrils or formed a toroidal structure, depending on the depth from the ACGS surface in the tubular diluted phase. The cells were uniformly distributed in ICGS. SAXS analysis suggests that collagen fibrils were remodeled by osteoblastic cells, and this remodeling process would be affected by the structure difference between ACGS and ICGS. These results suggest that we directly regulate cell-ECM interaction by the unique anisotropic and hierarchical structure of ACGS. The cell-gel composite presented in this study would promise an efficient scaffold material in tissue engineering.
|
| 巻・号 |
5(13)
|
| ページ |
5937-46
|
| 公開日 |
2013-7-10
|
| DOI |
10.1021/am303254e
|
| PMID |
23806015
|
| MeSH |
Animals
Anisotropy
Collagen / chemistry*
Extracellular Matrix / chemistry
Gels / chemistry
Mice
Microscopy, Confocal
Osteoblasts / cytology
Scattering, Small Angle
Tissue Engineering / instrumentation*
Tissue Scaffolds / chemistry*
|
| IF |
8.758
|
| 引用数 |
7
|
|
WOS 分野
|
MATERIALS SCIENCE, MULTIDISCIPLINARY
NANOSCIENCE & NANOTECHNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
