Reference - Detail
| RRC ID | 44503 |
|---|---|
| Author | Tamai M, Adachi E, Tagawa Y. |
| Title | Characterization of a liver organoid tissue composed of hepatocytes and fibroblasts in dense collagen fibrils. |
| Journal | Tissue Eng Part A |
| Abstract |
The adult liver is wrapped in a connective tissue sheet called the liver capsule, which consists of collagen fibrils and fibroblasts. In this study, we set out to construct a liver organoid tissue that would be comparable to the endogenous liver, using a bioreactor. In vitro liver organoid tissue was generated by combining collagen fibrils, fibroblasts, and primary murine hepatocytes or Hep G2 on a mesh of poly-lactic acid fabric using a bioreactor. Then, the suitability of this liver organoid tissue for transplantation was tested by implanting the constructs into partially hepatectomized BALB/cA-nu/nu mice. As determined by using scanning and transmission electron microscopes, the liver organoid tissues were composed of densely packed collagen fibrils with fibroblasts and aggregates of oval or spherical hepatocytes. Angiogenesis was induced after the transplantation, and blood vessels connected the liver organoid tissue with the surrounding tissue. Thus, a novel approach was applied to generate transplantable liver organoid tissue within a condensed collagen fibril matrix. These results suggested that a dense collagen network populated with fibroblasts can hold a layer of concentrated hepatocytes, providing a three-dimensional microenvrionment suitable for the reestablishment of cell-cell and cell-extracellular matrix (ECM) interactions, and resulting in the maintenance of their liver-specific functions. This liver organoid tissue may be useful for the study of intrahepatic functions of various cells, cytokines, and ECMs, and may fulfill the fundamental requirements of a donor tissue. |
| Volume | 19(21-22) |
| Pages | 2527-35 |
| Published | 2013-11-1 |
| DOI | 10.1089/ten.TEA.2012.0704 |
| PMID | 23815236 |
| PMC | PMC3807535 |
| MeSH | Animals Collagen / chemistry* Female Fibroblasts / cytology* Hep G2 Cells Hepatocytes / cytology* Humans Liver / cytology* Mice Mice, Inbred BALB C Microscopy, Electron, Transmission Organoids / cytology* Organoids / ultrastructure Pregnancy |
| IF | 3.508 |
| Times Cited | 10 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY CELL & TISSUE ENGINEERING CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 11 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | Hep G2 |