RRC ID 4451
Author King MC, Drivas TG, Blobel G.
Title A network of nuclear envelope membrane proteins linking centromeres to microtubules.
Journal Cell
Abstract In the fission yeast S. pombe, nuclei are actively positioned at the cell center by microtubules. Here, we show that cytoplasmic microtubules are mechanically coupled to the nuclear heterochromatin through proteins embedded in the nuclear envelope. This includes an integral outer nuclear membrane protein of the KASH family (Kms2) and two integral inner nuclear membrane proteins, the SUN-domain protein Sad1 and the previously uncharacterized protein Ima1. Ima1 specifically binds to heterochromatic regions and promotes the tethering of centromeric DNA to the SUN-KASH complex. In the absence of Ima1, or in cells harboring mutations in the centromeric Ndc80 complex, inefficient coupling of centromeric heterochromatin to Sad1 leads to striking defects in the ability of the nucleus to tolerate microtubule-dependent forces, leading to changes in nuclear shape, loss of spindle pole body components from the nuclear envelope, and partial dissociation of SUN-KASH complexes. This work highlights a framework for communication between cytoplasmic microtubules and chromatin.
Volume 134(3)
Pages 427-38
Published 2008-8-8
DOI 10.1016/j.cell.2008.06.022
PII S0092-8674(08)00774-5
PMID 18692466
PMC PMC2617791
MeSH Cell Cycle Proteins / metabolism* Cell Nucleus / metabolism Centromere / metabolism* Chromosomal Proteins, Non-Histone / metabolism Cytoskeleton / metabolism Heterochromatin / metabolism Membrane Proteins / metabolism Microtubules / metabolism* Nuclear Envelope / metabolism Schizosaccharomyces pombe Proteins / metabolism*
IF 31.398
Times Cited 116
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Yeast MKSP111