論文 - 詳細
| RRC ID | 4451 |
|---|---|
| 著者 | King MC, Drivas TG, Blobel G. |
| タイトル | A network of nuclear envelope membrane proteins linking centromeres to microtubules. |
| ジャーナル | Cell |
| Abstract |
In the fission yeast S. pombe, nuclei are actively positioned at the cell center by microtubules. Here, we show that cytoplasmic microtubules are mechanically coupled to the nuclear heterochromatin through proteins embedded in the nuclear envelope. This includes an integral outer nuclear membrane protein of the KASH family (Kms2) and two integral inner nuclear membrane proteins, the SUN-domain protein Sad1 and the previously uncharacterized protein Ima1. Ima1 specifically binds to heterochromatic regions and promotes the tethering of centromeric DNA to the SUN-KASH complex. In the absence of Ima1, or in cells harboring mutations in the centromeric Ndc80 complex, inefficient coupling of centromeric heterochromatin to Sad1 leads to striking defects in the ability of the nucleus to tolerate microtubule-dependent forces, leading to changes in nuclear shape, loss of spindle pole body components from the nuclear envelope, and partial dissociation of SUN-KASH complexes. This work highlights a framework for communication between cytoplasmic microtubules and chromatin. |
| 巻・号 | 134(3) |
| ページ | 427-38 |
| 公開日 | 2008-8-8 |
| DOI | 10.1016/j.cell.2008.06.022 |
| PII | S0092-8674(08)00774-5 |
| PMID | 18692466 |
| PMC | PMC2617791 |
| MeSH | Cell Cycle Proteins / metabolism* Cell Nucleus / metabolism Centromere / metabolism* Chromosomal Proteins, Non-Histone / metabolism Cytoskeleton / metabolism Heterochromatin / metabolism Membrane Proteins / metabolism Microtubules / metabolism* Nuclear Envelope / metabolism Schizosaccharomyces pombe Proteins / metabolism* |
| IF | 38.637 |
| 引用数 | 138 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | MKSP111 |