RRC ID 44513
Author Kobayashi H, Motoyoshi N, Itagaki T, Tabata K, Suzuki T, Inokuchi N.
Title The inhibition of human tumor cell proliferation by RNase Pol, a member of the RNase T1 family, from Pleurotus ostreatus.
Journal Biosci. Biotechnol. Biochem.
Abstract RNase Po1 is a guanylic acid-specific ribonuclease (a RNase T1 family RNase) from Pleurotus ostreatus. We determined the cDNA sequence encoding RNase Po1 and expressed RNase Po1 in Escherichia coli. A comparison of the enzymatic properties of RNase Po1 and RNase T1 indicated that the optimum temperature for RNase Po1 activity was 20 °C higher than that for RNase T1. An MTT assay indicated that RNase Po1 inhibits the proliferation of human neuroblastoma cells (IMR-32 and SK-N-SH) and human leukemia cells (Jurkat and HL-60). Furthermore, Hoechst 33342 staining showed morphological changes in HL-60 cells due to RNase Po1, and flow cytometry indicated the appearance of a sub-G1 cell population. The extent of these changes was dependent on the concentration of RNase Pol. We suggest that RNase Po1 induces apoptosis in tumor cells.
Volume 77(7)
Pages 1486-91
Published 2013
DOI 10.1271/bbb.130133
PII DN/JST.JSTAGE/bbb/130133
PMID 23832341
MeSH Amino Acid Sequence Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology* Apoptosis / drug effects Base Sequence Cell Cycle Checkpoints / drug effects Cell Proliferation / drug effects Cloning, Molecular HL-60 Cells Humans Jurkat Cells Molecular Sequence Data Pleurotus / enzymology* Pleurotus / genetics Ribonuclease T1 / chemistry Ribonuclease T1 / genetics Ribonuclease T1 / pharmacology*
IF 1.255
Times Cited 3
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells