RRC ID 44546
著者 Kwon HJ, Ohmiya Y.
タイトル Metabolomic analysis of differential changes in metabolites during ATP oscillations in chondrogenesis.
ジャーナル Biomed Res Int
Abstract Prechondrogenic condensation is a critical step for skeletal pattern formation. Recent studies reported that ATP oscillations play an essential role in prechondrogenic condensation. However, the molecular mechanism to underlie ATP oscillations remains poorly understood. In the present study, it was investigated how changes in metabolites are implicated in ATP oscillations during chondrogenesis by using capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS). CE-TOF-MS detected 93 cationic and 109 anionic compounds derived from known metabolic pathways. 15 cationic and 18 anionic compounds revealed significant change between peak and trough of ATP oscillations. These results implicate that glycolysis, mitochondrial respiration and uronic acid pathway oscillate in phase with ATP oscillations, while PPRP and nucleotides synthesis pathways oscillate in antiphase with ATP oscillations. This suggests that the ATP-producing glycolysis and mitochondrial respiration oscillate in antiphase with the ATP-consuming PPRP/nucleotide synthesis pathway during chondrogenesis.
巻・号 2013
ページ 213972
公開日 2013-1-1
DOI 10.1155/2013/213972
PMID 23878799
PMC PMC3708381
MeSH Adenosine Triphosphate / physiology* Biological Clocks / physiology* Cell Line Chondrocytes / physiology* Chondrogenesis / physiology* Gene Expression Regulation, Developmental / physiology* Humans Metabolome / physiology*
IF 2.276
引用数 4
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)