RRC ID 44555
著者 Arita T, Ichikawa D, Konishi H, Komatsu S, Shiozaki A, Shoda K, Kawaguchi T, Hirajima S, Nagata H, Kubota T, Fujiwara H, Okamoto K, Otsuji E.
タイトル Circulating long non-coding RNAs in plasma of patients with gastric cancer.
ジャーナル Anticancer Res
Abstract BACKGROUND:We examined the levels of long non-coding RNAs (lncRNAs) in the plasma of patients with gastric cancer to assess their clinical significance for diseases diagnosing and monitoring.
MATERIALS AND METHODS:We investigated the stability of plasma lncRNAs, and then confirmed the appropriateness of the lncRNA assay with a pre-amplification method. The levels of plasma lncRNAs, H19, HOX antisense intergenic RNA (HOTAIR), and metastasis associated lung adenocarcinoma transcript-1 (MALAT1), were then analyzed in patients with gastric cancer (GC) and healthy controls.
RESULTS:Plasma lncRNAs exhibited minimal gradual instability only under several severe conditions. Analysis showed that samples with pre-amplification had a higher level of linearity in the reverse transcription polymerase chain reaction (RT-PCR) assay than those without pre-amplification. Plasma H19 levels were significantly higher in patients than in healthy controls. Plasma H19 levels were significantly reduced in postoperative samples.
CONCLUSION:Circulating lncRNAs can be detectable in plasma, and the detection of circulating lncRNAs may provide new complementary tumor markers for gastric cancer.
巻・号 33(8)
ページ 3185-93
公開日 2013-8-1
PII 33/8/3185
PMID 23898077
MeSH Case-Control Studies Humans Postoperative Care Preoperative Care RNA Stability RNA, Long Noncoding / blood* Stomach Neoplasms / blood* Stomach Neoplasms / genetics* Stomach Neoplasms / surgery
IF 1.994
引用数 172
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 HGC-27(RCB0500)