論文 - 詳細
RRC ID | 44555 |
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著者 | Arita T, Ichikawa D, Konishi H, Komatsu S, Shiozaki A, Shoda K, Kawaguchi T, Hirajima S, Nagata H, Kubota T, Fujiwara H, Okamoto K, Otsuji E. |
タイトル | Circulating long non-coding RNAs in plasma of patients with gastric cancer. |
ジャーナル | Anticancer Res |
Abstract |
BACKGROUND:We examined the levels of long non-coding RNAs (lncRNAs) in the plasma of patients with gastric cancer to assess their clinical significance for diseases diagnosing and monitoring. MATERIALS AND METHODS:We investigated the stability of plasma lncRNAs, and then confirmed the appropriateness of the lncRNA assay with a pre-amplification method. The levels of plasma lncRNAs, H19, HOX antisense intergenic RNA (HOTAIR), and metastasis associated lung adenocarcinoma transcript-1 (MALAT1), were then analyzed in patients with gastric cancer (GC) and healthy controls. RESULTS:Plasma lncRNAs exhibited minimal gradual instability only under several severe conditions. Analysis showed that samples with pre-amplification had a higher level of linearity in the reverse transcription polymerase chain reaction (RT-PCR) assay than those without pre-amplification. Plasma H19 levels were significantly higher in patients than in healthy controls. Plasma H19 levels were significantly reduced in postoperative samples. CONCLUSION:Circulating lncRNAs can be detectable in plasma, and the detection of circulating lncRNAs may provide new complementary tumor markers for gastric cancer. |
巻・号 | 33(8) |
ページ | 3185-93 |
公開日 | 2013-8-1 |
PII | 33/8/3185 |
PMID | 23898077 |
MeSH | Case-Control Studies Humans Postoperative Care Preoperative Care RNA Stability RNA, Long Noncoding / blood* Stomach Neoplasms / blood* Stomach Neoplasms / genetics* Stomach Neoplasms / surgery |
IF | 1.994 |
引用数 | 172 |
WOS 分野 | ONCOLOGY |
リソース情報 | |
ヒト・動物細胞 | HGC-27(RCB0500) |