RRC ID 44557
Author Kim J, Sachdev P, Sidhu K.
Title Alginate microcapsule as a 3D platform for the efficient differentiation of human embryonic stem cells to dopamine neurons.
Journal Stem Cell Res
Abstract 100 folds after 2 weeks and at least 50% higher expression after 4 weeks respectively, compared to cells differentiated under conventional two-dimensional (2D) platform. The encapsulated TH(+) cells co-expressed mdDA neuronal markers, forkhead box protein A-2 (FOXA2) and pituitary homeobox-3 (PITX3) after 4 weeks and secreted approximately 60pg/ml/10(6) cells higher DA level when induced. We propose that the 3D platform facilitated an early onset of DA neuronal generation compared to that with conventional 2D system which also secretes more DA under potassium-induction. It is a very useful model to study the proliferation and directed differentiation of hESCs to various lineages, particularly to mdDA neurons. This 3D system also allows the separation of feeder cells from hESCs during the process of differentiation and also has potential for immune-isolation during transplantation studies.
Volume 11(3)
Pages 978-89
Published 2013-11
DOI 10.1016/j.scr.2013.06.005
PII S1873-5061(13)00078-0
PMID 23900167
MeSH Alginates / chemistry* Animals Biomarkers / metabolism Capsules / chemistry* Cell Culture Techniques Cell Line Cell Lineage Cell Proliferation Coculture Techniques Dopamine / metabolism Dopaminergic Neurons / cytology* Dopaminergic Neurons / enzymology Dopaminergic Neurons / metabolism Embryonic Stem Cells / cytology* Glucuronic Acid / chemistry Hepatocyte Nuclear Factor 3-beta / metabolism Hexuronic Acids / chemistry Homeodomain Proteins / metabolism Humans Mice Models, Biological Neurogenesis Transcription Factors / metabolism Tyrosine 3-Monooxygenase / metabolism
IF 3.902
Times Cited 16
Human and Animal Cells