論文 - 詳細
| RRC ID | 44558 |
|---|---|
| 著者 | Ozeki N, Yamaguchi H, Kawai R, Hiyama T, Nakata K, Mogi M, Nakamura H. |
| タイトル | Cytokines induce MMP-3-regulated proliferation of embryonic stem cell-derived odontoblast-like cells. |
| ジャーナル | Oral Dis |
| Abstract |
OBJECTIVES:Matrix metalloproteinase (MMP)-3 expression increases after pulpectomy and accelerates angiogenesis in rat dental pulp by an uncharacterised mechanism. Odontoblasts, a major component of dental pulp, could represent a therapeutic target. We investigated whether MMP-3 activity is induced by cytokines and/or is associated with cell proliferation and apoptosis in embryonic stem cell-derived odontoblast-like cells. MATERIALS AND METHODS:We used reverse transcriptase polymerase chain reaction, western blotting, an MMP-3 activity assay, a BrdU-cell proliferation enzyme-linked immunosorbent assay and DNA fragmentation analysis to evaluate siRNA-mediated downregulation of MMP-3 expression and activity, and any changes in the proliferative and apoptotic responses associated with this reduced expression. RESULTS:Pro-inflammatory cytokines (interleukin-1β, tumour necrosis factor-α and interferon-γ, at relatively low concentrations) induced MMP-3 mRNA and protein expression, and increased MMP-3 activity and cell proliferation, but not apoptosis. MMP-3 silencing produced a potent and significant suppression of cytokine-induced MMP-3 expression and activity, decreased cell proliferation and increased apoptosis. These effects were rescued by application of exogenous MMP-3. CONCLUSIONS:Our results suggest that pro-inflammatory cytokines induce MMP-3-regulated cell proliferation and anti-apoptosis effects in odontoblast-like cells derived from embryonic stem cells, in addition to their well-documented destructive role in inflammation. |
| 巻・号 | 20(5) |
| ページ | 505-13 |
| 公開日 | 2014-7-1 |
| DOI | 10.1111/odi.12165 |
| PMID | 23902456 |
| MeSH | Animals Apoptosis / physiology Blotting, Western Cell Division / physiology Cell Line Cell Proliferation* Cytokines / physiology* Embryonic Stem Cells / cytology* Matrix Metalloproteinase 3 / physiology* Mice Odontoblasts / cytology* Odontoblasts / drug effects Proteins |
| IF | 2.613 |
| 引用数 | 14 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| リソース情報 | |
| ヒト・動物細胞 | B6G-2(AES0003) |