論文 - 詳細
| RRC ID | 44576 |
|---|---|
| 著者 | Miyata S, Mizuno T, Koyama Y, Katayama T, Tohyama M. |
| タイトル | The endoplasmic reticulum-resident chaperone heat shock protein 47 protects the Golgi apparatus from the effects of O-glycosylation inhibition. |
| ジャーナル | PLoS One |
| Abstract |
The Golgi apparatus is important for the transport of secretory cargo. Glycosylation is a major post-translational event. Recognition of O-glycans on proteins is necessary for glycoprotein trafficking. In this study, specific inhibition of O-glycosylation (Golgi stress) induced the expression of endoplasmic reticulum (ER)-resident heat shock protein (HSP) 47 in NIH3T3 cells, although cell death was not induced by Golgi stress alone. When HSP47 expression was downregulated by siRNA, inhibition of O-glycosylation caused cell death. Three days after the induction of Golgi stress, the Golgi apparatus was disassembled, many vacuoles appeared near the Golgi apparatus and extended into the cytoplasm, the nuclei had split, and cell death assay-positive cells appeared. Six hours after the induction of Golgi stress, HSP47-knockdown cells exhibited increased cleavage of Golgi-resident caspase-2. Furthermore, activation of mitochondrial caspase-9 and ER-resident unfolded protein response (UPR)-related molecules and efflux of cytochrome c from the mitochondria to the cytoplasm was observed in HSP47-knockdown cells 24 h after the induction of Golgi stress. These findings indicate that (i) the ER-resident chaperon HSP47 protected cells from Golgi stress, and (ii) Golgi stress-induced cell death caused by the inhibition of HSP47 expression resulted from caspase-2 activation in the Golgi apparatus, extending to the ER and mitochondria. |
| 巻・号 | 8(7) |
| ページ | e69732 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0069732 |
| PII | PONE-D-13-04631 |
| PMID | 23922785 |
| PMC | PMC3726774 |
| MeSH | Animals Endoplasmic Reticulum / metabolism* Glycosylation Golgi Apparatus / metabolism* HSP47 Heat-Shock Proteins / metabolism* Mice Mitochondria / metabolism NIH 3T3 Cells |
| IF | 2.74 |
| 引用数 | 24 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH/3T3(RCB2767) |