論文 - 詳細
| RRC ID | 44607 |
|---|---|
| 著者 | Fukuda A, Shimada M, Nakadai T, Nishimura K, Hisatake K. |
| タイトル | Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene. |
| ジャーナル | PLoS One |
| Abstract |
The c-fos gene responds to extracellular stimuli and undergoes robust but transient transcriptional activation. Here we show that heterogeneous nuclear ribonucleoprotein R (hnRNP R) facilitates transcription reinitiation of the c-fos promoter in vitro in cooperation with Mediator. Consistently, hnRNP R interacts with the Scaffold components (Mediator, TBP, and TFIIH) as well as TFIIB, which recruits RNA polymerase II (Pol II) and TFIIF to Scaffold. The cooperative action of hnRNP R and Mediator is diminished by the cyclin-dependent kinase 8 (CDK8) module, which is comprised of CDK8, Cyclin C, MED12 and MED13 of the Mediator subunits. Interestingly, we find that the length of the G-free cassettes, and thereby their transcripts, influences the hnRNP R-mediated facilitation of reinitiation. Indeed, indicative of a possible role of the transcript in facilitating transcription reinitiation, the RNA transcript produced from the G-free cassette interacts with hnRNP R through its RNA recognition motifs (RRMs) and arginine-glycine-glycine (RGG) domain. Mutational analyses of hnRNP R indicate that facilitation of initiation and reinitiation requires distinct domains of hnRNP R. Knockdown of hnRNP R in mouse cells compromised rapid induction of the c-fos gene but did not affect transcription of constitutive genes. Together, these results suggest an important role for hnRNP R in regulating robust response of the c-fos gene. |
| 巻・号 | 8(8) |
| ページ | e72496 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0072496 |
| PII | PONE-D-12-25470 |
| PMID | 23967313 |
| PMC | PMC3742609 |
| MeSH | Animals Carrier Proteins / metabolism* Cell Line Cyclin C / metabolism Cyclin-Dependent Kinase 8 / metabolism Gene Expression Regulation Heterogeneous-Nuclear Ribonucleoproteins / chemistry Heterogeneous-Nuclear Ribonucleoproteins / metabolism* Mice Promoter Regions, Genetic Protein Binding Protein Interaction Domains and Motifs Proto-Oncogene Proteins c-fos / genetics* TATA-Box Binding Protein / metabolism Transcription Factor TFIIB / metabolism Transcription Initiation, Genetic* |
| IF | 2.74 |
| 引用数 | 5 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) |