RRC ID 44610
著者 Taniguchi M, Ikeda A, Nakamichi S, Ishiyama Y, Saitoh E, Kato T, Ochiai A, Tanaka T.
タイトル Antimicrobial activity and mechanism of action of a novel cationic α-helical octadecapeptide derived from heat shock protein 70 of rice.
ジャーナル Peptides
Abstract Hsp70(241-258), an octadecapeptide derived from the heat shock protein 70 (Hsp70) of rice (Oryza sativa L. japonica), is a novel cationic α-helical antimicrobial peptide (AMP) that contains four lysine, two arginine, and two histidine residues. The antimicrobial activity of Hsp70(241-258) against Porphyromonas gingivalis, a periodontal pathogen, and Candida albicans, an opportunistic fungal pathogen, was quantitatively evaluated using a chemiluminescence method that measures ATP derived from viable cells. The 50% growth-inhibitory concentrations of Hsp70(241-258) against P. gingivalis and C. albicans cells were 63 μM and 70 μM, respectively. Hsp70(241-258) had little or no hemolytic activity even at 1mM, and showed negligible cytotoxicity up to 300 μM. The degrees of calcein leakage from large unilamellar vesicles, which mimic the membranes of Gram-negative bacteria, and 3,3'-dipropylthiadicarbocyanine iodide release from P. gingivalis cells induced by the addition of Hsp70(241-258) increased in a concentration-dependent manner. When Hsp70(241-258) was added to calcein-acetoxymethyl ester-loaded C. albicans cells, calcein release from the cells increased in a concentration-dependent manner. Flow cytometric analysis also showed that the percentages of C. albicans cells stained with propidium iodide, a DNA-intercalating dye, increased as the concentration of Hsp70(241-258) added was increased. Therefore, Hsp70(241-258) appears to exhibit antimicrobial activity against P. gingivalis and C. albicans through membrane disruption. These results suggest that Hsp70(241-258) could be useful as a safe and potent AMP against P. gingivalis and C. albicans in many fields of health care, especially in the control of oral infections.
巻・号 48
ページ 147-55
公開日 2013-10-1
DOI 10.1016/j.peptides.2013.08.011
PII S0196-9781(13)00282-9
PMID 23973864
MeSH Amino Acid Sequence Anti-Infective Agents / chemistry Anti-Infective Agents / pharmacology Antimicrobial Cationic Peptides / chemistry Antimicrobial Cationic Peptides / pharmacology* Candida albicans / drug effects* Cell Survival / drug effects Flow Cytometry Gingival Diseases / drug therapy HSP70 Heat-Shock Proteins / chemistry HSP70 Heat-Shock Proteins / pharmacology* Hemolysis Humans Oryza / chemistry Porphyromonas gingivalis / drug effects* Structure-Activity Relationship
IF 2.843
引用数 14
WOS 分野 PHARMACOLOGY & PHARMACY ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)