RRC ID 44626
Author Tsuchiya K, Nitta N, Sonoda A, Otani H, Takahashi M, Murata K, Shiomi M, Tabata Y, Nohara S.
Title Atherosclerotic imaging using 4 types of superparamagnetic iron oxides: new possibilities for mannan-coated particles.
Journal Eur J Radiol
Abstract PURPOSE:We used magnetic resonance imaging (MRI) and histologic techniques to compare the uptake by the rabbit atherosclerotic wall of 4 types of superparamagnetic iron oxide (SPIO) particles, i.e. SPIO, mannan-coated SPIO (M-SPIO), ultrasmall SPIO (USPIO), and mannan-coated USPIO (M-USPIO).
MATERIALS AND METHODS:All experimental protocols were approved by our institutional animal experimentation committee. We intravenously injected 12 Watanabe heritable hyperlipidemic rabbits with one of the 4 types of SPIO (0.8 mmol Fe/kg). Two other rabbits served as the control. The rabbits underwent in vivo contrast-enhanced magnetic resonance angiography (MRA) before- and 5 days after these injections; excised aortae were subjected to in vitro MRI. In the in vivo and in vitro studies we assessed the signal intensity of the vessels at identical regions of interest (ROI) and calculated the signal-to-noise ratio (SNR). For histologic assessment we evaluated the iron-positive regions in Prussian blue-stained specimens.
RESULTS:There were significant differences in iron-positive regions where M-USPIO>USPIO, M-SPIO>SPIO, USPIO>SPIO (p<0.05) but not between M-USPIO and M-SPIO. The difference between the pre- and post-injection SNR was significantly greater in rabbits treated with M-USPIO than USPIO and in rabbits injected with M-SPIO than SPIO (p<0.05). On in vitro MRI scans SNR tended to be lower in M-USPIO- and M-SPIO- than USPIO- and SPIO-treated rabbits (p<0.1).
CONCLUSION:Histologic and imaging analysis showed that mannan-coated SPIO and USPIO particles were taken up more readily by the atherosclerotic rabbit wall than uncoated SPIO and USPIO.
Volume 82(11)
Pages 1919-25
Published 2013-11-1
DOI 10.1016/j.ejrad.2013.07.017
PII S0720-048X(13)00387-2
PMID 24001603
MeSH Animals Atherosclerosis / metabolism* Atherosclerosis / pathology* Contrast Media / chemical synthesis Contrast Media / pharmacokinetics Dextrans* / chemistry Dextrans* / pharmacokinetics Magnetic Resonance Imaging / methods* Magnetite Nanoparticles* / chemistry Mannans / chemistry Mannans / pharmacokinetics* Nanocapsules / chemistry Nanocapsules / ultrastructure Particle Size Rabbits Reproducibility of Results Sensitivity and Specificity
IF 2.687
Times Cited 14
WOS Category RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Resource
Human and Animal Cells J774.1(RCB0434)