Reference - Detail
| RRC ID | 44642 |
|---|---|
| Author | Yamazoe H, Sugiyama Y, El Omri A, Hagihara Y, Okada T. |
| Title | Facile immunostaining and labeling of nonadherent cells using a microfluidic device to entrap the cells. |
| Journal | J Biosci Bioeng |
| Abstract |
We fabricated a microfluidic device for the entrapment of nonadherent cells. Solution exchange was easily performed by introducing the solution into the cell-trapping microchannel. Immunostaining and labeling of the cell membrane of THP-1 cells were demonstrated using this device, which does not require cumbersome repetition of centrifugation and resuspension steps. |
| Volume | 117(3) |
| Pages | 375-8 |
| Published | 2014-3-1 |
| DOI | 10.1016/j.jbiosc.2013.08.008 |
| PII | S1389-1723(13)00309-5 |
| PMID | 24035233 |
| MeSH | Actins / metabolism Cell Adhesion Cell Line Cell Membrane / metabolism* Cell Separation / instrumentation Cell Separation / methods* Fluorescent Dyes Humans Microfluidic Analytical Techniques / instrumentation* Monocytes / cytology Monocytes / immunology Monocytes / metabolism |
| IF | 2.366 |
| Times Cited | 3 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| Human and Animal Cells | THP-1(RCB1189) |