RRC ID 44643
Author Suzuki K, Anada T, Miyazaki T, Miyatake N, Honda Y, Kishimoto KN, Hosaka M, Imaizumi H, Itoi E, Suzuki O.
Title Effect of addition of hyaluronic acids on the osteoconductivity and biodegradability of synthetic octacalcium phosphate.
Journal Acta Biomater
Abstract The present study was designed to investigate whether three sodium hyaluronic acid (HyA) medical products, Artz(®), Suvenyl(®) and a chemically modified derivative of sodium HyA Synvisc(®), can be used as suitable vehicles for an osteoconductive octacalcium phosphate (OCP). OCP granules (300-500 μm diameter) were mixed with these sodium HyAs with molecular weights of 90 × 10(4) (Artz(®)), 190 × 10(4) (Suvenyl(®)) and 600 × 10(4) (Synvisc(®)) (referred to as HyA90, HyA190 and HyA600, respectively). OCP-HyA composites were injected using a syringe into a polytetrafluoroethylene ring, placed on the subperiosteal region of mouse calvaria for 3 and 6 weeks, and then bone formation was assessed by histomorphometry. The capacity of the HyAs for osteoclast formation from RAW264 cells with RANKL was examined by TRAP staining in vitro. Bone formation was enhanced by the OCP composites with HyA90 and HyA600, compared to OCP alone, through enhanced osteoclastic resorption of OCP. HyA90 and HyA600 facilitated in vitro osteoclast formation. The results suggest that the osteoconductive property of OCP was accelerated by the HyAs-associated osteoclastic resorption of OCP, and therefore that HyA/OCP composites are attractive bone substitutes which are injectable and bioactive materials.
Volume 10(1)
Pages 531-43
Published 2014-1-1
DOI 10.1016/j.actbio.2013.09.005
PII S1742-7061(13)00453-4
PMID 24035888
MeSH Acid Phosphatase / metabolism Alkaline Phosphatase / metabolism Animals Bone Regeneration / drug effects* Calcium Phosphates / pharmacology* Cell Line Humans Hyaluronic Acid / pharmacology* Implants, Experimental Isoenzymes / metabolism Male Mice Mice, Inbred ICR Molecular Weight Osteoblasts / cytology Osteoblasts / drug effects Osteoblasts / enzymology Osteocalcin / metabolism Osteoclasts / cytology Osteoclasts / drug effects Osteoclasts / enzymology Osteogenesis / drug effects RANK Ligand / pharmacology Skull / drug effects Spectroscopy, Fourier Transform Infrared Tartrate-Resistant Acid Phosphatase Viscosity / drug effects X-Ray Diffraction
IF 6.638
Times Cited 28
Human and Animal Cells