論文 - 詳細
| RRC ID | 44665 |
|---|---|
| 著者 | Asayama S, Matsuda K, Negishi Y, Kawakami H. |
| タイトル | Intracellular co-delivery of zinc ions and plasmid DNA for enhancing gene transfection activity. |
| ジャーナル | Metallomics |
| Abstract |
Zinc ions, methylated poly(1-vinylimidazole) (PVIm-Me) and plasmid DNA (pDNA) have formed ternary complexes for gene delivery. The resulting Zn-PVIm-Me-pDNA complexes have delivered both Zn(2+) ions and pDNA inside cells, leading to the nuclear translocation of the pDNA. By use of the pDNA containing a nuclear protein, NF-κB, binding sequence, the intracellular co-delivery of Zn(2+) ions and pDNA has enhanced gene expression. These results suggest that the intracellular Zn(2+) ions delivered by Zn-PVIm-Me-pDNA complexes activated the NF-κB, enhancing the nuclear translocation of the pDNA. In conclusion, it has been demonstrated that the Zn-PVIm-Me-pDNA complex is capable of enhancing the gene transfection activity by a synergic effect of the PVIm-Me and the co-delivered intracellular Zn(2+) ions. |
| 巻・号 | 6(1) |
| ページ | 82-7 |
| 公開日 | 2014-1-1 |
| DOI | 10.1039/c3mt00226h |
| PMID | 24084762 |
| MeSH | Active Transport, Cell Nucleus Cell Nucleus / metabolism Coordination Complexes / chemistry Coordination Complexes / metabolism DNA / chemistry DNA / genetics DNA / metabolism* Hep G2 Cells Humans Imidazoles / chemistry Intracellular Space / metabolism Microscopy, Confocal Molecular Structure NF-kappa B / genetics NF-kappa B / metabolism Plasmids / chemistry Plasmids / genetics Plasmids / metabolism* Polyvinyls / chemistry Transfection / methods* Zinc / chemistry Zinc / metabolism* |
| IF | 3.796 |
| 引用数 | 11 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |