Reference - Detail
RRC ID | 44689 |
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Author | Sugimoto K, Ichikawa-Tomikawa N, Satohisa S, Akashi Y, Kanai R, Saito T, Sawada N, Chiba H. |
Title | The tight-junction protein claudin-6 induces epithelial differentiation from mouse F9 and embryonic stem cells. |
Journal | PLoS One |
Abstract |
During epithelialization, cell adhesions and polarity must be established to maintain tissue assemblies and separate the biological compartments in the body. However, the molecular basis of epithelial morphogenesis, in particular, a role of cell adhesion molecules in epithelial differentiation from stem cells, remains unclear. Here, we show that the stable and conditional expression of a tight-junction protein, claudin-6 (Cldn6), triggers epithelial morphogenesis in mouse F9 stem cells. We also demonstrate that Cldn6 induces the expression of other tight-junction and microvillus molecules including Cldn7, occludin, ZO-1α+, and ezrin/radixin/moesin-binding phosphoprotein50. These events were inhibited by attenuation of Cldn6 using RNA interference or the C-terminal half of Clostridium Perfringens enterotoxin. Furthermore, similar results were obtained in mouse embryonic stem cells. Thus, we have uncovered that the Cldn6 functions as a novel cue to induce epithelial differentiation. |
Volume | 8(10) |
Pages | e75106 |
Published | 2013-1-1 |
DOI | 10.1371/journal.pone.0075106 |
PII | PONE-D-13-22287 |
PMID | 24116027 |
PMC | PMC3792957 |
MeSH | Animals Cell Differentiation / physiology* Claudins / genetics Claudins / metabolism* Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism Epithelial Cells / cytology* Epithelial Cells / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Mice Occludin / genetics Occludin / metabolism Zonula Occludens-1 Protein / genetics Zonula Occludens-1 Protein / metabolism |
IF | 2.74 |
Times Cited | 12 |
WOS Category | CELL BIOLOGY |
Resource | |
Human and Animal Cells | BRC4(AES0008) |