RRC ID 44696
著者 Kubota H, Matsumoto H, Higashida M, Murakami H, Nakashima H, Oka Y, Okumura H, Yamamura M, Nakamura M, Hirai T.
タイトル Eicosapentaenoic acid modifies cytokine activity and inhibits cell proliferation in an oesophageal cancer cell line.
ジャーナル Anticancer Res
Abstract BACKGROUND/AIM:The present study investigated the effect of eicosapentaenoic acid (EPA) on nuclear factor-kappa B (NF-κB) activation, inflammatory interleukin-6 (IL-6) production, and cell proliferation in a human oesophageal carcinoma cell line (TE-1).
MATERIALS AND METHODS:Lipopolysaccharide (LPS)-induced IL-6 production in TE-1 cells in the presence or absence of EPA was determined using enzyme-linked immunosorbent assay (ELISA). The proliferation of TE-1 cells was determined by the WST-1 assay. TE-1 cells were stained with Hoechst 33342 and propidium iodide to observe apoptosis. Immunohistochemical staining of NF-κB in TE-1 cells was performed.
RESULTS:LPS increased IL-6 production in TE-1 cells, and EPA treatment prevented this effect. EPA treatment inhibited NF-κB activation and induced apoptosis of TE-1 cells.
CONCLUSION:EPA inhibits NF-κB activation and IL-6 production in oesophageal cancer cells, their inducing apoptosis. These effects of EPA may be of benefit in improving the outcome of cancer surgery.
巻・号 33(10)
ページ 4319-24
公開日 2013-10-1
PII 33/10/4319
PMID 24122998
MeSH Anti-Inflammatory Agents / pharmacology* Antineoplastic Agents / pharmacology Apoptosis / drug effects Apoptosis / immunology Cell Line, Tumor Cell Nucleus / metabolism Cell Proliferation / drug effects* Cell Shape / drug effects Eicosapentaenoic Acid / pharmacology* Esophageal Neoplasms Humans Interleukin-6 / metabolism* Lipopolysaccharides / pharmacology NF-kappa B / metabolism Protein Transport
IF 1.994
引用数 13
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 TE-1(RCB1894)