論文 - 詳細
RRC ID | 44696 |
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著者 | Kubota H, Matsumoto H, Higashida M, Murakami H, Nakashima H, Oka Y, Okumura H, Yamamura M, Nakamura M, Hirai T. |
タイトル | Eicosapentaenoic acid modifies cytokine activity and inhibits cell proliferation in an oesophageal cancer cell line. |
ジャーナル | Anticancer Res |
Abstract |
BACKGROUND/AIM:The present study investigated the effect of eicosapentaenoic acid (EPA) on nuclear factor-kappa B (NF-κB) activation, inflammatory interleukin-6 (IL-6) production, and cell proliferation in a human oesophageal carcinoma cell line (TE-1). MATERIALS AND METHODS:Lipopolysaccharide (LPS)-induced IL-6 production in TE-1 cells in the presence or absence of EPA was determined using enzyme-linked immunosorbent assay (ELISA). The proliferation of TE-1 cells was determined by the WST-1 assay. TE-1 cells were stained with Hoechst 33342 and propidium iodide to observe apoptosis. Immunohistochemical staining of NF-κB in TE-1 cells was performed. RESULTS:LPS increased IL-6 production in TE-1 cells, and EPA treatment prevented this effect. EPA treatment inhibited NF-κB activation and induced apoptosis of TE-1 cells. CONCLUSION:EPA inhibits NF-κB activation and IL-6 production in oesophageal cancer cells, their inducing apoptosis. These effects of EPA may be of benefit in improving the outcome of cancer surgery. |
巻・号 | 33(10) |
ページ | 4319-24 |
公開日 | 2013-10-1 |
PII | 33/10/4319 |
PMID | 24122998 |
MeSH | Anti-Inflammatory Agents / pharmacology* Antineoplastic Agents / pharmacology Apoptosis / drug effects Apoptosis / immunology Cell Line, Tumor Cell Nucleus / metabolism Cell Proliferation / drug effects* Cell Shape / drug effects Eicosapentaenoic Acid / pharmacology* Esophageal Neoplasms Humans Interleukin-6 / metabolism* Lipopolysaccharides / pharmacology NF-kappa B / metabolism Protein Transport |
IF | 1.994 |
引用数 | 13 |
WOS 分野 | ONCOLOGY |
リソース情報 | |
ヒト・動物細胞 | TE-1(RCB1894) |