RRC ID 44730
Author Sekine S, Shimada Y, Nagata T, Sawada S, Yoshioka I, Matsui K, Moriyama M, Omura T, Osawa S, Shibuya K, Hashimoto I, Watanabe T, Hojo S, Hori R, Okumura T, Yoshida T, Tsukada K.
Title Role of aquaporin-5 in gallbladder carcinoma.
Journal Eur Surg Res
Abstract BACKGROUND/PURPOSE:Aquaporins (AQPs) are important in controlling bile formation. However, the exact role in human gallbladder carcinogenesis has not yet been defined.
METHODS:AQP-5-expressing gallbladder carcinoma (GBC) cell lines (NOZ) were transfected with anti-AQP-5 small interfering RNA (siRNA). Growth, migration, invasion assay, and drug susceptibility tests were performed. Next, microRNA (miRNA) expression was analyzed by miRNA oligo chip (3D-Gene®). AQP-5 and AQP-5-related miRNA target gene expressions were also analyzed using tissue microarray (TMA) in 44 GBC samples.
RESULTS:Treatment with AQP-5 siRNA decreased cell proliferation, migration, and invasion. On the other hand, those cells increased IC50 of gemcitabine. By performing miRNA assays, miR-29b, -200a, and -21 were shown to be highly overexpressed in cells treated with AQP-5 siRNA NOZ. When focusing on miR-21, phosphatase and tensin homolog (PTEN) was found to be a target of miR-21. In the TMA, AQP-5/PTEN coexpression was significantly associated with the depth of invasion and MIB-1 index (p = 0.003, 0.010). Survival of patients with a high AQP-5/PTEN coexpression was longer than that of patients with a low coexpression (p = 0.003).
CONCLUSIONS:Our result suggested that miR-21 and PTEN may contribute to the role of AQP-5 in GBC. AQP-5 and PTEN cascades are favorable biomarkers of GBC.
Volume 51(3-4)
Pages 108-17
Published 2013
DOI 10.1159/000355675
PII 000355675
PMID 24217644
MeSH Adult Aged Aquaporin 5 / genetics Aquaporin 5 / physiology* Cell Line, Tumor Cell Movement Female Gallbladder Neoplasms / etiology* Gallbladder Neoplasms / genetics Gallbladder Neoplasms / mortality Gallbladder Neoplasms / pathology Humans Male MicroRNAs / analysis Middle Aged Neoplasm Invasiveness PTEN Phosphohydrolase / analysis PTEN Phosphohydrolase / physiology RNA, Messenger / analysis Tissue Array Analysis
IF 1.629
Times Cited 11
Human and Animal Cells