RRC ID 44733
Author Matsumoto Y, Cao E, Ueoka R.
Title Growth inhibition by novel liposomes including trehalose surfactant against hepatocarcinoma cells along with apoptosis.
Journal Anticancer Res.
Abstract Novel liposomes composed of L-α-dimyristoylphosphatidylcholine (DMPC) and trehalose surfactant (DMTreCn) were produced by the method of sonication in buffer solution. The thickness of fixed aqueous layer of DMTreCn was larger than that of DMPC liposomes and increased in a dose-dependent manner. The remarkable inhibitory effects of DMTreCn on the growth of human hepatocellular carcinoma (HCC) (Hep-G2 and HuH-7) cells were obtained along with apoptosis, without affecting the growth of normal cells. DMTreCn induced apoptosis of Hep-G2 and HuH-7 cells through the activation of caspase-3, 8 and 9. Release of cytochrome c from mitochondria and activation of Bcl-2 family protein (BAX) were recorded, indicating that DMTreCn induced apoptosis of Hep-G2 and HuH-7 cells through mitochondrial pathway via BAX. It is noteworthy that the remarkable inhibitory effects of DMTreCn on the growth of human HCC cells were obtained along with apoptosis for the first time.
Volume 33(11)
Pages 4727-40
Published 2013-11
PII 33/11/4727
PMID 24222107
MeSH Apoptosis / drug effects* Carcinoma, Hepatocellular / drug therapy Carcinoma, Hepatocellular / metabolism Carcinoma, Hepatocellular / pathology* Caspase 3 / metabolism Cell Membrane / drug effects Cell Membrane / metabolism* Cytochromes c / metabolism Flow Cytometry Humans Liposomes* Liver Neoplasms / drug therapy Liver Neoplasms / metabolism Liver Neoplasms / pathology* Membrane Potential, Mitochondrial / drug effects Proto-Oncogene Proteins c-bcl-2 / metabolism Surface-Active Agents / pharmacology* Trehalose / pharmacology* Tumor Cells, Cultured bcl-2-Associated X Protein / metabolism
IF 1.865
Times Cited 3
WOS Category ONCOLOGY
Resource
Human and Animal Cells