Reference - Detail
| RRC ID | 44751 |
|---|---|
| Author | Tabuchi Y, Sugahara Y, Ikegame M, Suzuki N, Kitamura K, Kondo T. |
| Title | Genes responsive to low-intensity pulsed ultrasound in MC3T3-E1 preosteoblast cells. |
| Journal | Int J Mol Sci |
| Abstract |
Although low-intensity pulsed ultrasound (LIPUS) has been shown to enhance bone fracture healing, the underlying mechanism of LIPUS remains to be fully elucidated. Here, to better understand the molecular mechanism underlying cellular responses to LIPUS, we investigated gene expression profiles in mouse MC3T3-E1 preosteoblast cells exposed to LIPUS using high-density oligonucleotide microarrays and computational gene expression analysis tools. Although treatment of the cells with a single 20-min LIPUS (1.5 MHz, 30 mW/cm(2)) did not affect the cell growth or alkaline phosphatase activity, the treatment significantly increased the mRNA level of Bglap. Microarray analysis demonstrated that 38 genes were upregulated and 37 genes were downregulated by 1.5-fold or more in the cells at 24-h post-treatment. Ingenuity pathway analysis demonstrated that the gene network U (up) contained many upregulated genes that were mainly associated with bone morphology in the category of biological functions of skeletal and muscular system development and function. Moreover, the biological function of the gene network D (down), which contained downregulated genes, was associated with gene expression, the cell cycle and connective tissue development and function. These results should help to further clarify the molecular basis of the mechanisms of the LIPUS response in osteoblast cells. |
| Volume | 14(11) |
| Pages | 22721-40 |
| Published | 2013-11-18 |
| DOI | 10.3390/ijms141122721 |
| PII | ijms141122721 |
| PMID | 24252911 |
| PMC | PMC3856087 |
| MeSH | 3T3 Cells Animals Cell Proliferation / radiation effects Gene Expression Regulation / genetics* Gene Expression Regulation / radiation effects Mice Osteoblasts / diagnostic imaging Osteoblasts / metabolism* Transcriptome / genetics* Transcriptome / radiation effects Ultrasonic Therapy* Ultrasonography |
| IF | 4.556 |
| Times Cited | 8 |
| WOS Category | CHEMISTRY, MULTIDISCIPLINARY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |