RRC ID 44753
Author Miyauchi E, Ogita T, Miyamoto J, Kawamoto S, Morita H, Ohno H, Suzuki T, Tanabe S.
Title Bifidobacterium longum alleviates dextran sulfate sodium-induced colitis by suppressing IL-17A response: involvement of intestinal epithelial costimulatory molecules.
Journal PLoS ONE
Abstract Although some bacterial strains show potential to prevent colitis, their mechanisms are not fully understood. Here, we investigated the anti-colitic mechanisms of Bifidobacterium longum subsp. infantis JCM 1222(T), focusing on the relationship between interleukin (IL)-17A secreting CD4(+) T cells and intestinal epithelial costimulatory molecules in mice. Oral administration of JCM 1222(T) to mice alleviated dextran sulfate sodium (DSS)-induced acute colitis. The expression of type 1 helper T (Th1)- and IL-17 producing helper T (Th17)-specific cytokines and transcriptional factors was suppressed by JCM 1222(T) treatment. Intestinal epithelial cells (IECs) from colitic mice induced IL-17A production from CD4(+) T cells in a cell-cell contact-dependent manner, and this was suppressed by oral treatment with JCM 1222(T). Using blocking antibodies for costimulatory molecules, we revealed that epithelial costimulatory molecules including CD80 and CD40, which were highly expressed in IECs from colitic mice, were involved in IEC-induced IL-17A response. Treatment of mice and intestinal epithelial cell line Colon-26 cells with JCM 1222(T) decreased the expression of CD80 and CD40. Collectively, these data indicate that JCM 1222(T) negatively regulate epithelial costimulatory molecules, and this effect might be attributed, at least in part, to suppression of IL-17A in DSS-induced colitis.
Volume 8(11)
Pages e79735
Published 2013
DOI 10.1371/journal.pone.0079735
PII PONE-D-13-27794
PMID 24255712
PMC PMC3821848
MeSH Animals B7-1 Antigen / genetics B7-1 Antigen / metabolism B7-2 Antigen / genetics B7-2 Antigen / metabolism Bifidobacterium / metabolism* CD40 Antigens / genetics CD40 Antigens / metabolism Colitis / chemically induced Colitis / genetics Colitis / immunology Colitis / metabolism* Colitis / microbiology Cytokines / metabolism Dextran Sulfate / adverse effects Female Gene Expression Regulation Interleukin-17 / metabolism* Intestinal Mucosa / immunology Intestinal Mucosa / metabolism Intestinal Mucosa / pathology Mice Th17 Cells / immunology Th17 Cells / metabolism Transcription Factors / genetics Transcription Factors / metabolism
IF 2.766
Times Cited 15
WOS Category GASTROENTEROLOGY & HEPATOLOGY
Resource
Human and Animal Cells