論文 - 詳細
| RRC ID | 44758 |
|---|---|
| 著者 | Goshima F, Esaki S, Luo C, Kamakura M, Kimura H, Nishiyama Y. |
| タイトル | Oncolytic viral therapy with a combination of HF10, a herpes simplex virus type 1 variant and granulocyte-macrophage colony-stimulating factor for murine ovarian cancer. |
| ジャーナル | Int J Cancer |
| Abstract |
Ovarian cancer is the most frequent cause of gynecological cancer-related mortality as a majority of patients are diagnosed at an advanced stage with intraperitoneal dissemination because of the absence of initial symptoms. Granulocyte-macrophage colony-stimulating factor (GM-CSF) plays an important role in the maturation of specialized antigen-presenting cells. In this study, we utilized a herpes simplex virus (HSV) amplicon expressing murine GM-CSF combined with HF10 (mGM-CSF amplicon), a highly attenuated HSV type 1 strain functioning as a helper virus to strengthen anti-tumor immune response, for the treatment of ovarian cancer with intraperitoneal dissemination. A mouse ovarian cancer cell line, OV2944-HM-1 (HM-1), was intraperitoneally injected, following which HF10 only or the mGM-CSF amplicon was injected intraperitoneally three times. HF10 injection prolonged survival and decreased intraperitoneal dissemination, but to a lesser extent than the mGM-CSF amplicon. Although HF10 replication was not observed in HM-1 cells, expression of VP5, a late gene coding the major capsid protein of HSV, was detected. Moreover, mGM-CSF production was detected in transfected HM-1 cells. Immunohistochemical staining revealed the infiltration of CD4- and CD8-positive cells into the peritoneal tumor(s). A significantly increased CD4+ T cell concentration was observed in the spleen. Murine splenic cells after each treatment were stimulated with HM-1 cells, and the strongest immune response was observed in the mice that received mGM-CSF amplicon injections. These results suggested that the mGM-CSF amplicon is a promising agent for the treatment of advanced ovarian cancer with intraperitoneal dissemination. |
| 巻・号 | 134(12) |
| ページ | 2865-77 |
| 公開日 | 2014-6-15 |
| DOI | 10.1002/ijc.28631 |
| PMID | 24265099 |
| MeSH | Animals CD4-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / immunology Capsid Proteins / biosynthesis Cell Line, Tumor Cell Movement / immunology Chlorocebus aethiops Female Genetic Vectors Granulocyte-Macrophage Colony-Stimulating Factor / genetics Granulocyte-Macrophage Colony-Stimulating Factor / immunology* Herpesvirus 1, Human / genetics* Herpesvirus 1, Human / immunology Interferon-gamma / metabolism Mice Mice, Inbred C57BL Neoplasm Transplantation Oncolytic Virotherapy / methods* Ovarian Neoplasms / immunology* Ovarian Neoplasms / therapy* Survival Tumor Necrosis Factor-alpha / metabolism Vero Cells |
| IF | 5.145 |
| 引用数 | 22 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OV2944-HM-1(RCB1483) |