| RRC ID |
44775
|
| 著者 |
Hayashi K, Pack CG, Sato MK, Mouri K, Kaizu K, Takahashi K, Okada Y.
|
| タイトル |
Viscosity and drag force involved in organelle transport: investigation of the fluctuation dissipation theorem.
|
| ジャーナル |
Eur Phys J E Soft Matter
|
| Abstract |
We observed the motion of an organelle transported by motor proteins in cells using fluorescence microscopy. Particularly, among organelles, the mitochondria in PC12 cells were studied. A mitochondrion was dragged at a constant speed for several seconds without pausing. We investigated the fluctuation dissipation theorem for this constant drag motion by comparing it with the motion of Brownian beads that were incorporated into the cells by an electroporation method. We estimated the viscosity value inside cells from the diffusion coefficients of the beads. Then the viscosity value obtained by using the beads was found to be slightly lower than that obtained from the diffusion coefficient for the organelle motion via the Einstein relation. This discrepancy indicates the violation of the Einstein relation for the organelle motion.
|
| 巻・号 |
36(12)
|
| ページ |
136
|
| 公開日 |
2013-12-1
|
| DOI |
10.1140/epje/i2013-13136-6
|
| PMID |
24297312
|
| MeSH |
Animals
Facilitated Diffusion*
Mitochondria / metabolism*
Models, Biological
Motion
PC12 Cells
Rats
Viscosity
|
| IF |
1.812
|
| 引用数 |
10
|
|
WOS 分野
|
PHYSICS, APPLIED
MATERIALS SCIENCE, MULTIDISCIPLINARY
POLYMER SCIENCE
CHEMISTRY, PHYSICAL
|
| リソース情報 |
| ヒト・動物細胞 |
|
