RRC ID 44775
Author Hayashi K, Pack CG, Sato MK, Mouri K, Kaizu K, Takahashi K, Okada Y.
Title Viscosity and drag force involved in organelle transport: investigation of the fluctuation dissipation theorem.
Journal Eur Phys J E Soft Matter
Abstract We observed the motion of an organelle transported by motor proteins in cells using fluorescence microscopy. Particularly, among organelles, the mitochondria in PC12 cells were studied. A mitochondrion was dragged at a constant speed for several seconds without pausing. We investigated the fluctuation dissipation theorem for this constant drag motion by comparing it with the motion of Brownian beads that were incorporated into the cells by an electroporation method. We estimated the viscosity value inside cells from the diffusion coefficients of the beads. Then the viscosity value obtained by using the beads was found to be slightly lower than that obtained from the diffusion coefficient for the organelle motion via the Einstein relation. This discrepancy indicates the violation of the Einstein relation for the organelle motion.
Volume 36(12)
Pages 136
Published 2013-12
DOI 10.1140/epje/i2013-13136-6
PMID 24297312
MeSH Animals Facilitated Diffusion* Mitochondria / metabolism* Models, Biological Motion PC12 Cells Rats Viscosity
IF 1.686
Times Cited 10
WOS Category PHYSICS, APPLIED MATERIALS SCIENCE, MULTIDISCIPLINARY POLYMER SCIENCE CHEMISTRY, PHYSICAL
Resource
Human and Animal Cells