RRC ID 44780
Author Ohtsu N, Hirano M, Arai H.
Title Response of osteoblast-like MC3T3-E1 cells on bioactive titanium fabricated by a chemical treatment process using a calcium-phosphate slurry.
Journal J Biomed Mater Res A
Abstract We recently developed a chemical treatment process using a calcium-phosphate slurry for fabricating new layers consisting of hydroxyapatite and titanium dioxide (TiO2) on titanium (Ti) substrate. In this study, the response of osteoblast-like MC3T3-E1 cells on Ti substrate treated with a calcium-phosphate slurry was investigated to elucidate its behavior in a biological environment. The cellular adhesiveness and proliferation capacity did not differ significantly between the treated and untreated Ti substrates, suggesting that the slurry treatment did not cause cytotoxicity. The slurry treatment did not affect the increase in alkaline phosphatase activity after the induction of cell differentiation, whereas it was found to be significantly advantageous for the calcification behavior on the slurry-treated Ti substrate. In consequence, the hard-tissue compatibility of Ti is expected to be improved by the chemical treatment process using a calcium-phosphate slurry.
Volume 102(11)
Pages 3838-45
Published 2014-11
DOI 10.1002/jbm.a.35053
PMID 24307316
MeSH Alkaline Phosphatase / metabolism Animals Calcification, Physiologic* Cell Differentiation* Cell Line Coated Materials, Biocompatible* / chemical synthesis Coated Materials, Biocompatible* / chemistry Durapatite / chemistry* Materials Testing Mice Osteoblasts / cytology Osteoblasts / metabolism* Titanium / chemistry*
IF 3.221
Times Cited 5
Human and Animal Cells