論文 - 詳細
| RRC ID | 44794 |
|---|---|
| 著者 | Miyanokoshi M, Tanaka T, Tamai M, Tagawa Y, Wakasugi K. |
| タイトル | Expression of the rodent-specific alternative splice variant of tryptophanyl-tRNA synthetase in murine tissues and cells. |
| ジャーナル | Sci Rep |
| Abstract |
Tryptophanyl-tRNA synthetase (TrpRS) catalyzes the aminoacylation of tRNA(Trp). mRNA of a rodent-specific alternative splice variant of TrpRS (SV-TrpRS), which results in the inclusion of an additional hexapeptide at the C-terminus of full-length TrpRS (FL-TrpRS), has been identified in murine embryonic stem (ES) cells. In the present study, we evaluated the expression of mouse TrpRS mRNA by real-time reverse transcription PCR. We show that SV-TrpRS and FL-TrpRS mRNAs are highly expressed in murine ES cells, embryo, spleen, lung, liver and uterus, and that the relative expression of SV-TrpRS compared to FL-TrpRS is significantly less in the brain. Moreover, we found that interferon-γ increases the expression of TrpRS in a mouse cell line. These results provide the first evidence for tissue-specific expression and alternative splicing of mouse TrpRS. |
| 巻・号 | 3 |
| ページ | 3477 |
| 公開日 | 2013-12-11 |
| DOI | 10.1038/srep03477 |
| PII | srep03477 |
| PMID | 24327169 |
| PMC | PMC3858792 |
| MeSH | Alternative Splicing* Animals Cell Line Embryonic Stem Cells / metabolism Gene Expression Regulation* / drug effects Interferon-gamma / pharmacology Mice Organ Specificity RNA Isoforms RNA, Messenger / genetics Species Specificity Tryptophan-tRNA Ligase / genetics* |
| IF | 3.998 |
| 引用数 | 3 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) Hepa1–6(RCB1638) |